▎ 摘　　要

NOVELTY - Preparing graphene oxide polystyrene fluorescent microspheres involves taking 5 mg amino polystyrene microspheres, washing with organic solvent for 1-3 times, and then washing them the surfactant solution for 1-3 times, and then adding 100-500 mu l surfactant solution under stirring to disperse to obtain liquid A. The surfactant solution and another organic solvent are separately mixed and emulsified on an ultrasonic cell disruptor to obtain an emulsion. 500-1000 mu l emulsion is added to the obtained solution A to obtain solution B. 50-300 mu l swelling aid is added to the solution B in a vertical constant temperature oscillator at 30-45 degrees C for 15-20 hours to obtain solution C. The europium chelate is weighed into a dichloromethane solution, and then mixed the dichloromethane solution of the europium chelate with the surfactant solution to obtain D liquid. USE - Method for preparing graphene oxide polystyrene fluorescent microspheres. ADVANTAGE - The method prepares the graphene oxide polystyrene fluorescent microspheres in a simple, cost-effective and eco-friendly manner with uniform particle size, good dispersibility, high fluorescence intensity, and good stability under different pHs. DETAILED DESCRIPTION - Preparing graphene oxide polystyrene fluorescent microspheres involves taking 5 mg amino polystyrene microspheres, washing with organic solvent for 1-3 times, and then washing them the surfactant solution for 1-3 times, and then adding 100-500 mu l surfactant solution under stirring to disperse to obtain liquid A. The surfactant solution and another organic solvent are separately mixed and emulsified on an ultrasonic cell disruptor to obtain an emulsion. 500-1000 mu l emulsion is added to the obtained solution A to obtain solution B. 50-300 mu l swelling aid is added to the solution B in a vertical constant temperature oscillator at 30-45 degrees C for 15-20 hours to obtain solution C. The europium chelate is weighed into a dichloromethane solution, and then mixed the dichloromethane solution of the europium chelate with the surfactant solution to obtain D liquid. The obtained liquid D is added to liquid C in a vertical constant temperature shaker at 30-45 degrees C for 3-8 hours, and then washed to obtain processed amino poly styrene microspheres. The treated amino polystyrene microspheres are dispersed in distilled water to prepare a solution of 10-15 mg/ml, sonicated for 25-35 minutes to obtain liquid E. The graphene oxide nanosheets and disperse in distilled water to prepare F solution, which is poured into E solution into a three-necked flask under mechanical stirring for 2-5 hours at 50 degrees C to obtain the graphene oxide polystyrene fluorescent microspheres.