▎ 摘　　要

NOVELTY - High strength polysaccharide-inorganic nanoparticle composite column is prepared by adding different inorganic nano-particles with a certain concentration in the polysaccharide water solution to mix uniformly to obtain the water phase, mixing the surfactant and water-insoluble organic solvent, uniformly mixing and heating to a certain temperature as the oil phase under the condition of mechanical stirring, dispersing the oil phase into the water phase, emulsifying for a certain time to form stable oil-in-water (O/W) emulsion; transferring the obtained emulsion to stainless steel or glass column pipe with rubber plug at one end by injector, using ethanol peristaltic pump to deionized water the organic solvent; and utilizing peristaltic pump to circulate the mixed solution of chemical cross-linking agent and alkali into the obtained whole column, sequentially ethanol deionized water ethanol the whole column to obtain polysaccharide-inorganic nano particle composite monolithic colum USE - High strength polysaccharide-inorganic nanoparticle composite column. ADVANTAGE - The composite column has wide application space in the enzyme immobilization, cell culture and fast separation and purification field of biological macromolecule. DETAILED DESCRIPTION - High strength polysaccharide-inorganic nanoparticle composite column is prepared by adding different inorganic nano-particles with a certain concentration in the polysaccharide water solution to mix uniformly to obtain the water phase, mixing the surfactant and water-insoluble organic solvent, uniformly mixing and heating to a certain temperature as the oil phase under the condition of mechanical stirring, dispersing the oil phase into the water phase, emulsifying for a certain time to form stable oil-in-water (O/W) emulsion; transferring the obtained emulsion to stainless steel or glass column pipe with rubber plug at one end by injector, soaking the column pipe filled with emulsion into 0.3M sodium sulphate aqueous solution for a period of time at room temperature, cooling for 12 hours at 4 degrees centigrade, using ethanol peristaltic pump to deionized water the organic solvent and obtain surfactant polysaccharide-inorganic nano-particle monolithic column; and utilizing peristaltic pump to circulate the mixed solution of chemical cross-linking agent and alkali into the obtained whole column, carrying out chemical cross-linking reaction at a certain temperature, after reacting for a period of time, and sequentially ethanol deionized water ethanol the whole column to obtain polysaccharide-inorganic nano particle composite monolithic column.