▎ 摘　　要

NOVELTY - An ultra-sensitive ascorbic acid biosensing material preparation method involves introducing foamed nickel and high purity methane in an atmosphere of hydrogen and argon under heating, and cooling to obtain a nickel/graphene three-dimensional network. The sample is placed in concentrated hydrochloric acid to give a self-supporting three-dimensional network of graphene. Electrochemical deposition of copper particles is performed in a uniform graphene surface and in potassium thiosulfate and sodium hydroxide solution to obtain a three-dimensional copper oxide nano-graphene biosensor material. USE - Method for preparing a three-dimensional copper oxide based ultra-sensitive ascorbic acid biosensing material. ADVANTAGE - The method enables preparing the biosensing material in a simple and economical manner with excellent electrochemical sensing performance, high yield and good reproducibility, and facilitating accurate detection of ascorbic acid. DETAILED DESCRIPTION - An ultra-sensitive ascorbic acid biosensing material preparation method involves introducing foamed nickel and high purity methane in an atmosphere of hydrogen and argon under heating at 1000 degrees C for 10 minutes to adsorb carbon source, and rapidly cooling to room temperature to obtain a nickel/graphene three-dimensional network. The sample is placed in concentrated hydrochloric acid corrosion of nickel stencil to give a self-supporting three-dimensional network of grapheme. Electrochemical deposition of copper particles is performed in a uniform graphene surface and ultimately in potassium thiosulfate and sodium hydroxide solution to obtain a three-dimensional copper oxide nano-graphene biosensor material.