▎ 摘　　要

NOVELTY - Preparation of electrochemical luminescence biosensor involves treating glassy carbon electrode with alumina polishing powder, dripping multiwalled carbon nanotubes/palladium nano cube solution to electrode surface, drying, rinsing the electrode surface with ultrapure water, drying, adding thrombin nucleic acid aptamer solution and bovine serum albumin, blocking non-specific binding sites, rinsing using ultrapure water, drying, dripping biocleavable polyrotaxane-plasmid DNA-silver/graphene quantum dot solution, heat-preserving, immersing in thrombin solution and heat-preserving. USE - Preparation of electrochemical luminescence biosensor used for detecting thrombin. ADVANTAGE - The method efficiently and economically provides electrochemical luminescence biosensor having improved electroluminescent stability, by simple method. The electrochemical luminescence biosensor efficiently detects thrombin with high sensitivity. DETAILED DESCRIPTION - Preparation of electrochemical luminescence biosensor involves successively treating glassy carbon electrode having diameter of 4 mm with 1 mu m, 0.3 mu m and 0.05 mu m alumina polishing powder, rinsing with ultrapure water, dripping 6 mu L 0.5-1.5 mg/mL multiwalled carbon nanotubes/palladium nano cube solution to the electrode surface, drying at room temperature, rinsing the electrode surface with ultrapure water, drying, adding 10 mu L 0.5-1.5 mu mol/L thrombin nucleic acid aptamer solution and 3 L 1% bovine serum albumin, blocking non-specific binding sites, rinsing using ultrapure water, drying in refrigerator at 4 degrees C, dripping 10 mu L of biocleavable polyrotaxane-plasmid DNA-silver/graphene quantum dot solution, heat-preserving at 37 degrees C for 1-3 hours, placing in refrigerator at 4 degrees C, drying, immersing resultant electrode in 1x 10-14-1x 10-11 mol/L different concentrations of thrombin solution, and heat-preserving at 37 degrees C for 40 minutes.