▎ 摘　　要

NOVELTY - Solid nanometer hole-fluorescence resonance energy transfer composite detection method involves using fluorescence microscope to focus on plane of solid nanometer hole; using paired fluorescent group to mark analyte molecule; collecting fluorescence resonance energy transfer signal between analyte molecules through hole current signal and paired fluorescent groupsfluorescent groups to mark the analyte molecule when analyte molecule passes through nanometer hole; transmitting fluorescence resonant energy transfer signals between through-hole current signal of analyter molecule and fluorescence group; matching characteristic light in time electric signal, composite analyzing molecular conformation information. USE - Solid nanometer hole-fluorescence resonance energy transfer composite detection method used in system (claimed) for single molecule conformation detection of biological molecules, DNA and RNA. ADVANTAGE - The solid nano-hole-fluorescence resonance energy transfer composite detection method is more effectively further distinguish micro-conformational difference between biomolecules, suitable for multiple analyte detection and different analyte mixed detection. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is also included for a solid nanometer hole-fluorescence resonance energy transfer composite detection system, which comprises silicon nitride solid nanometer hole chip, silver electrode membrane clamp system, confocal microscope, and multifunctional data collecting system, where silicon nitride solid nano-hole chip, which is placed on glass slide; nano-hole lower gap, which contains cis-side solution; nano-hole, which is instilled with trans-side solution, where two side solution fixed respectively silver electrode, silver electrode through front end amplifier amplifying signal, and connected with membrane clamp system; glass slide, which is placed under confocal microscope objective; excitation source makes laser focus on plane of solid nano-hole to detect fluorescence resonance energy transfer signal of molecule passing through plane; excitation light respectively donor fluorescent group, and acceptor fluorescent group, which is detected by two photomultiplier detectors, and converted into electric signal; fluorescence resonance energy transfer signal of analyte molecule to detected, which is collected, and processed by data collection system through electric signal of solid nanometer hole.