• 专利标题:   Preparation of magnetic graphene oxide immobilized enzyme used for corn starch extraction includes taking graphene oxide, adding ethanol solution, mixing, reacting, adjusting pH, performing magnetic separation, washing and drying.
  • 专利号:   CN108179143-A
  • 发明人:   LIU Q, SHI J, MA Y, YANG J, MENG Q, WANG B, MA H, LI Q, GONG W, CAI L, ZHENG L, GAO G
  • 专利权人:   SHANDONG BIOLOGY INST
  • 国际专利分类:   C12N011/14, C08B030/04
  • 专利详细信息:   CN108179143-A 19 Jun 2018 C12N-011/14 201855 Pages: 5 Chinese
  • 申请详细信息:   CN108179143-A CN10239162 22 Mar 2018
  • 优先权号:   CN10239162

▎ 摘  要

NOVELTY - Preparation of magnetic graphene oxide immobilized enzyme comprises taking graphene oxide (GO), adding 30-50% ethanol solution, performing ultrasonic dispersion for 0.5 hour, adding ferrous salt and iron salt, mixing for 30 minutes, reacting at 50 degrees C to reflux temperature for 1-2 hours, adjusting pH using ammonia to 11, mixing for 1-2 hours, performing magnetic separation, washing, drying graphene oxide/iron oxide (GO/Fe3O4) composite magnetic material, placing GO/Fe3O4 composite magnetic material and papain in phosphate buffer solution, placing in ultrasound for 30-45 minutes, performing magnetic separation, washing and drying magnetic graphene oxide immobilized enzyme. USE - Method of preparing magnetic graphene oxide immobilized enzyme used for corn starch extraction (claimed). ADVANTAGE - The method has simple process steps and high corn starch yield, can recycle magnetic graphene oxide immobilized enzyme 10 times and does not change rate and product quality.