▎ 摘　　要

NOVELTY - A reduced graphene oxide/tannin palladium/platinum-palladium composite nano material is prepared by taking graphene, placing it in distilled water and ultrasonically dispersing to obtain a graphene suspension, adding an ascorbic acid to the suspension, stirring, centrifuging, and removing the supernatant to obtain reduced graphene oxide, weighing tannin palladium into reduced graphene oxide solution, ultrasonication, making reduced graphene oxide/tannin palladium suspension, adding hydrogen tetrachloroplatinate(II) and palladium(II) nitrate, adding acetic acid, stirring, centrifuging, removing the supernatant, and washing the precipitate with ultrapure water and centrifuging to obtain reduced graphene oxide/tannin palladium/platinum-palladium composite nano material. USE - Reduced graphene oxide/tannin palladium/platinum-palladium composite nano material. ADVANTAGE - The reduced graphene oxide/tannin palladium/platinum-palladium composite nano material has high specific surface area, electron transfer efficiency, load characteristic and high sensitivity. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is also included for a method for detecting 1,5-anhydroglucitol, which involves placing a screen printing electrode in a sulfuric acid solution for cyclic voltammetry scanning to obtain an activated screen printing electrode, and rinsing with pure water, performing a constant potential deposition of gold on the activated screen-printed electrode, and washing, pipetting the reduced graphene oxide/tannin palladium/platinum-palladium composite nano material suspension, adding to the surface of the pretreated electrode, and incubating the electrode in the incubator to obtain reduced graphene oxide/tannin palladium/platinum-palladium composite nano material modified working electrode, adding PROD solution to the surface of the reduced graphene oxide/tannin palladium/platinum-palladium composite nanomaterial modified electrode and incubating it in air, which is the electrochemical biosensing interface of the constructed 1,5-anhydroglucitol, adding the 1,5-anhydroglucitol standard solution to the 1,5-anhydroglucitol electrochemical biosensing interface, and putting it in the incubator for a period of time to obtain a 1,5-anhydroglucitol electrochemical biosensor, immersing the working electrode into the PBS solution, scanning with a differential pulse voltammetry for utilizing an electrochemical workstation, and recording the response current value of the sensor, drawing a working curve and calculating the minimum detection limit of the method according to the relationship between the current response value of the sensor and the concentration of 1,5-anhydroglucitol, adding a certain amount of the actual sample to be tested at the 1,5-anhydroglucitol electrochemical biosensing interface, and incubating it in the incubator for a period of time and washing the PBS solution to obtain the working electrode, and drying, putting the working electrode into the PBS solution, and utilizing the differential pulse voltammetry scan of the electrochemical workstation to record the peak current, and obtaining the concentration of 1,5-AG in the actual sample to be tested according to the standard curve.