▎ 摘　　要

NOVELTY - Preparing electrochemical aptamer for detecting acetamiprid comprises placing the Indium tin oxide (ITO) electrode in the acetone, mixing ethanol and water for ultrasonic cleaning, adding reduced graphene oxide nanocomposite (Au-PEDOT/rGO) coating drop on the cleaning surface of an ITO electrode, and drying in the oven; immersing the modified base material of ITO electrode in water, ethanol, and cetyl trimethylammonium bromide (CTAB), ammonia aqueous solution and tetraethyl orthosilicate (TEOS) in the mixture solution, the mesoporous silica films (MSFs) growth is controlled and rinsing. USE - The method is useful for preparing electrochemical aptamer for detecting acetamiprid (claimed). ADVANTAGE - The sensor has simple design and is easy to operate, and enables rapid and sensitive detection of acetamiprid. DETAILED DESCRIPTION - Preparing electrochemical aptamer for detecting acetamiprid comprises placing the Indium tin oxide (ITO) electrode in the acetone, mixing ethanol and water for ultrasonic cleaning for 15 minutes, adding 10 mu l reduced graphene oxide nanocomposite (Au-PEDOT/rGO) coating drop on the cleaning surface of an ITO electrode, and drying in the oven at 45 degrees C; immersing the modified base material of ITO electrode in 35 ml water, 15 ml ethanol, and 0.08 g cetyl trimethylammonium bromide (CTAB), 5 mu l 25% ammonia aqueous solution and 40 mu l tetraethyl orthosilicate (TEOS) in the mixture solution, the mesoporous silica films (MSFs) growth is controlled under 60 degrees C for 48 hours, rinsing the ITO electrode with ethanol and water, and overnight drying at 60 degrees C; placing the MSFs modified electrode into 0.1 mol/l hydrochloric acid in ethanol for 5 minutes, removing the CTAB surfactant under stirring, placing the MSFs modified ITO electrode into 4 ml ethanol solution of 5% (3-aminopropyl)triethoxysilane (APTES) for 12 hours, lightly shaking to ensure the surface of the MSFs for further functionalizing with amino, ITO electrode of MSFs modified after amination into a methylene blue concentration of 10-5 mol/l tris-hydrochloric acid solution (10 mmol/l, pH 7.4), and oscillating overnight at room temperature, then, the 10 aptamer mu l 10-8 mol/l solution to load the methylene blue MSFs modified electrode surface, incubating at room temperature for 2 hours to obtain aptamers blocked MSFs modified electrode; incubating with adapter body, washing the modified ITO electrode with 10 mmol/L tris-hydrochloric acid buffer solution (pH 7.4), removing non-specific adsorption at room temperature for the 2 MSFs modified electrode into aptamer-terminated 10 mu l contains a certain volume of acetamiprid in buffer solution, and washing by buffer solution for removing and specifically binds acetamiprid aptamer. An INDEPENDENT CLAIM is also included for detecting acetamiprid comprises (i) containing 10 ml phosphate buffer saline solution at pH 7 in the electrolytic cell, the ITO of the modification is a working electrode, an silver/silver chloride (Ag/AgCl) electrode as reference electrode, platinum electrode as counter electrode, the experiment on the CHI842C electrochemical comprehensive test instrument, auxiliary computer software for collecting and processing experiment data; performing differential pulse scanning in the range of -0.5 to 0.1 V potential, recording differential pulse voltammogram, measuring the peak current value Ip0 without adding acetamiprid, adding different concentration of acetamiprid to obtain the corresponding peak current value Ipx, calculating the peak current difference Delta Ip ( Delta Ip=Ip0-Ipx), log lgc DIp/Ip0 and Acetamiprid concentration have good linear relation, linear equation is (Ip/Ip0=0.49258+0.05731lgc, c is the concentration, unit is mol/l), Ip is the peak current difference, the unit is mA, linear correlation coefficient R=0.99477, acetamiprid concentration linear range is 10-8 to 10-13 mol/l, sensor to acetamiprid pesticide detection limit is 3.4x 10-14 mol/l, and (ii) combining with the linear relation of acetamiprid sample of unknown concentration is measured for calculating the acetamiprid concentration containing 10 ml phosphate buffer saline solution at pH 7 in the electrolytic cell, the ITO of the modification is working electrode, Ag/AgCl electrode as reference electrode, platinum electrode as counter electrode, the experiment on the CHI842C electrochemical comprehensive test instrument, auxiliary computer software for collecting and processing experiment data, adding a certain volume of the solution to be tested, performing differential pulse scanning in the range from -0.5 to 0.1 V potential, recording differential pulse voltammogram obtained peak current value Ip, Ip is substituted into the equation, and calculating.