▎ 摘　　要

NOVELTY - Preparing enzyme immobilization graphene oxide/chitosan aerogel comprises (i) performing Hummers modification method to prepare graphene oxide stock solution, performing centrifugal washing and stripping the obtained graphene oxide stock solution using a centrifuge to obtain graphene oxide slurry and freeze drying to obtain graphene oxide, where the method adopts chemical cross-linking combined with freeze drying; (ii) taking graphene oxide and dispersing in deionized water for ultrasonic treatment to obtain graphene oxide dispersion; (iii) taking the chitosan powder and dissolving in an acetic acid solution with acetic acid concentration of 4 wt.%, obtaining the chitosan acetic acid solution, and adding the graphene oxide dispersion into the chitosan acetic acid solution and mixing; (iv) stirring vigorously after ultrasonic de-foaming to form graphene oxide/chitosan solid gel; (v) mixing and crosslinking the graphene oxide/chitosan solid gel using glutaraldehyde solution. USE - The method is useful for preparing enzyme immobilization graphene oxide/chitosan aerogel. ADVANTAGE - The method is easy to covalently bind with enzyme and easy to be modified by grafting, and improves the physical and chemical properties of the original enzyme, and ensures excellent stability in large temperature. DETAILED DESCRIPTION - Preparing enzyme immobilization graphene oxide/chitosan aerogel comprises (i) performing Hummers modification method to prepare graphene oxide stock solution, performing centrifugal washing and stripping the obtained graphene oxide stock solution using a centrifuge to obtain graphene oxide slurry and freeze drying to obtain graphene oxide, where the method adopts chemical cross-linking combined with freeze drying; (ii) taking graphene oxide and dispersing in deionized water for ultrasonic treatment to obtain graphene oxide dispersion; (iii) taking the chitosan powder and dissolving in an acetic acid solution with acetic acid concentration of 4 wt.%, obtaining the chitosan acetic acid solution, and adding the graphene oxide dispersion into the chitosan acetic acid solution and mixing uniformly; (iv) stirring vigorously after ultrasonic de-foaming to form graphene oxide/chitosan solid gel; (v) mixing and crosslinking the graphene oxide/chitosan solid gel using glutaraldehyde solution, and aging the resulting product; and (vi) drying in a vacuum freezing environment.