• 专利标题:   Preparation of antibacterial magnetic nanozyme used in in-vitro antibacterial application, involves preparing reduced graphene oxide-loaded ferroferric oxide magnetic nanozyme, and modifying bacterial broad-spectrum affinity agent-engineered mannose lectin on surface of magnetic nanozyme.
  • 专利号:   CN115944727-A
  • 发明人:   FENG B, GAO W, XUE Y, YU S
  • 专利权人:   UNIV NINGBO
  • 国际专利分类:   A61K033/26, A61K033/44, A61K041/00, A61K047/62, A61P031/04, C01B032/194
  • 专利详细信息:   CN115944727-A 11 Apr 2023 A61K-041/00 202342 Chinese
  • 申请详细信息:   CN115944727-A CN11592531 13 Dec 2022
  • 优先权号:   CN11592531

▎ 摘  要

NOVELTY - Preparation of antibacterial magnetic nanozyme involves preparing reduced graphene oxide-loaded ferroferric oxide magnetic nanozyme by in-situ reduction method, and modifying bacterial broad-spectrum affinity agent-engineered mannose lectin on the surface of the magnetic nanozyme. USE - Preparation of antibacterial magnetic nanozyme used in in-vitro antibacterial application (claimed). ADVANTAGE - Preparation method utilizes broad-spectrum bacterial affinity of Fc-MBL and physical property of graphene to enhance the material's ability to trap bacteria, and utilizes excellent peroxidase-like activity of ferroferric oxide and photothermal conversion performance of reduced graphene oxide to achieve material with synergistic antimicrobial therapy of photothermal therapy (PTT) and chemodynamic therapy (CDT) effects. The antibacterial magnetic nanozyme can achieve rapid enrichment of bacteria within 5 minutes, and catalyze the conversion of low concentration of hydrogen peroxide into hydroxy, which is toxic to bacteria, exhibits excellent photothermal effect under near infrared irradiation, enhance the antibacterial effect of CDT, and has excellent biocompatibility and broad-spectrum antibacterial ability to achieve rapid and effective antimicrobial treatment at low concentration of 40 μg/mL and milder temperature condition of 47℃. DETAILED DESCRIPTION - Preparation of antibacterial magnetic nanozyme involves preparing reduced graphene oxide-loaded ferroferric oxide magnetic nanozyme (rGO:Fe3O4) by in-situ reduction method, and modifying bacterial broad-spectrum affinity agent-engineered mannose lectin (Fc-MBL) on the surface of the magnetic nanozyme.