▎ 摘　　要

NOVELTY - Preparation of antibacterial magnetic nanozyme involves preparing reduced graphene oxide-loaded ferroferric oxide magnetic nanozyme by in-situ reduction method, and modifying bacterial broad-spectrum affinity agent-engineered mannose lectin on the surface of the magnetic nanozyme. USE - Preparation of antibacterial magnetic nanozyme used in in-vitro antibacterial application (claimed). ADVANTAGE - Preparation method utilizes broad-spectrum bacterial affinity of Fc-MBL and physical property of graphene to enhance the material's ability to trap bacteria, and utilizes excellent peroxidase-like activity of ferroferric oxide and photothermal conversion performance of reduced graphene oxide to achieve material with synergistic antimicrobial therapy of photothermal therapy (PTT) and chemodynamic therapy (CDT) effects. The antibacterial magnetic nanozyme can achieve rapid enrichment of bacteria within 5 minutes, and catalyze the conversion of low concentration of hydrogen peroxide into hydroxy, which is toxic to bacteria, exhibits excellent photothermal effect under near infrared irradiation, enhance the antibacterial effect of CDT, and has excellent biocompatibility and broad-spectrum antibacterial ability to achieve rapid and effective antimicrobial treatment at low concentration of 40 μg/mL and milder temperature condition of 47℃. DETAILED DESCRIPTION - Preparation of antibacterial magnetic nanozyme involves preparing reduced graphene oxide-loaded ferroferric oxide magnetic nanozyme (rGO:Fe3O4) by in-situ reduction method, and modifying bacterial broad-spectrum affinity agent-engineered mannose lectin (Fc-MBL) on the surface of the magnetic nanozyme.