▎ 摘　　要

NOVELTY - Electrochemical aptamer sensor for rapid detection of ofloxacin, is prepared by loading aptamer (P-APT) on surface of glassy carbon electrode modified by zirconium-based metal-organic framework (UIO-67)/reduced graphene oxide (rGO) composite. USE - Electrochemical aptamer sensor used for rapid detection of ofloxacin (claimed). ADVANTAGE - The sensor has simple operation, no complicated pretreatment steps, no organic solvent extraction and washing process, low cost and fast detection speed. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for: (1) preparation of the electrochemical aptamer sensor for rapid detection of ofloxacin, which involves: (a) preparing metal organic framework material (UIO-67); (b) preparing reduced graphene oxide (rGO) by L-ascorbic acid (LAA) reduction method; (c) dispersing the UIO-67 in aqueous solution, sonicating for 20 minutes, weighing reduced graphene oxide powder and adding to obtained solution, adding appropriate amount of LAA, putting in a water bath at 80℃ for 24 hours, vacuum filtering, washing with deionized water and ethanol, respectively, placing in an ethanol solution overnight, washing again with ethanol, placing in vacuum oven to dry to obtain a composite material of reduced graphene oxide and UIO-67; (d) grinding and polishing the glassy carbon electrode, rinsing the electrode surface with deionized water, ultrasonically cleaning polished electrode tip in deionized water and drying in vacuum, dispersing the UIO-67/rGO composite material in phosphate buffered saline buffer solution to obtain a dispersion, dripping the dispersion to surface of polished glassy carbon electrode, drying in vacuum oven to obtain a glassy carbon electrode modified by the composite material UIO-67/rGO; and (e) loading the aptamer P-APT solution on surface of the glassy carbon electrode modified by UIO-67/rGO composite material, and drying in vacuum oven; and (2) method for rapid detection of ofloxacin, which involves: (a) using the electrochemical aptamer sensor as working motor, platinum wire electrode as counter electrode and saturated calomel electrode as three-electrode system composed of reference motor, dripping the sample to be tested into electrolytic cell containing potassium ferricyanide solution, measuring by differential pulse voltammetry under scanning speed of 50 mV/second and potential range of -0.3-0.7 V, and quantitatively capturing ofloxacin in the sample to be tested on surface of the sensor and detecting through generated electrochemical signal.