▎ 摘　　要

NOVELTY - Determining Pb2 + (lead(2+)) by using quantum dots of graphene activated by surface-enhanced Raman spectroscopy comprises e.g. adding 5 mu l, 10 mu l, 20 mu l, 50 mu l, 100 mu l, 1 mu mol/l Pb2 + standard solution into five graduated test tubes, adding 10 mu l 0.15 mu mol/l lead aptamer and graphene quantum dots to each graduated tube, mixing, allowing to stand for 10 minutes, adding hydrochloric acid, 175 mu l 0.5 mol/l glucose and 100 mu l 84 mu mol/l trichlorogold-hydrochloride, mixing, adding 1 ml double distilled water, heating, removing test tube and then quenching reaction with ice-water. USE - The method is useful for determining lead(2+) by using quantum dots of graphene activated by surface-enhanced Raman spectroscopy. ADVANTAGE - The method has simple process, high sensitivity, and is stable. DETAILED DESCRIPTION - Determining Pb2 + (lead(2+)) by using quantum dots of graphene activated by surface-enhanced Raman spectroscopy comprises (i) adding 5 mu l, 10 mu l, 20 mu l, 50 mu l, 100 mu l, 1 mu mol/l Pb2 + standard solution into five graduated test tubes, adding 10 mu l 0.15 mu mol/l lead aptamer and 100 mu l 0.5 mg/l graphene quantum dots to each graduated tube, mixing, allowing to stand for 10 minutes, adding 40 mu l 0.01 mol/l hydrochloric acid, 175 mu l 0.5 mol/l glucose and 100 mu l 84 mu mol/l trichlorogold-hydrochloride, mixing, adding 1 ml double distilled water, heating at 75 degrees C under water bath for 18 minutes, removing test tube, quenching reaction with ice-water, adding 50 mu l 10 mu ol/l victoria blue solution B i.e. VBB and adding 1.5 ml double distilled water, (ii) using method of step (i) without adding Pb2 + standard solution to obtain blank control solution system, (iii) adding prepared Pb2 standard solution system and blank control solution into quartz cuvette on the DXR smart Raman spectrometer, setting the instrument parameters laser power to 3 mW, slit is 50 mu m, acquisition time is 3 seconds, scanning the surface enhanced Raman spectra of the system, determining 1615 cm-1 at surface enhanced Raman peak intensity value of I, then determining blank control solution surface enhanced Raman peak intensity is I0 and calculating Delta I = I-I0, (iv) taking Delta I as working curve of relationship between Pb2 + concentration to obtain linear regression equation as Delta I1615 cm-1 = 12.62C + 0.35, where unit of Pb2 + concentration C is nmol/l, linear range of determination is 3.33-66.67 nmol/l, and detection limit is 1.33 nmol/l, (v) taking pond water from suburbs of Guilin, stream water, filtering with filter paper, measure amount of filtrate, adding test sample of step (i) to Pb2 + standard solution replacing by test sample and measuring Delta I sample = I sample-I0, and (vi) calculating content of the sample Pb2 +, content of Pb2 + in pond water is 5.6 nmol/l, content of Pb2 + in stream water is 1.9 nmol/l.