▎ 摘　　要

NOVELTY - Preparation of biamino chitosan-graphene oxide hybrid material involves (1) dissolving O-carboxymethyl chitosan in 2-(N-morpholine)ethanesulfonic acid (MES) buffer solution of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide/N-hydroxysuccinimide (EDC/NHS) to obtain solution (A), and stirring to react in an ice bath, (2) mixing the reaction solution obtained in the step (1) with the MES buffer solution of mono-tert-butoxycarbonyl-protected diamine to obtain solution (B), adding sodium hydroxide to control the pH to 6-8, and stirring the reaction at room temperature, (3) adding hydrochloric acid to adjust the pH of the solution to 4.9-5.1, (4) dispersing the mono-tert-butoxycarbonyl-protected diamino chitosan derivative in methanol, (5) mixing a graphene oxide butyl acetate suspension with an condensing agent, and (6) adding bisamino chitosan derivatives to the mixture (C), and washing with deionized water, ethanol and acetone cyclically, and then drying. USE - Preparation of biamino chitosan-graphene oxide hybrid material for constructing plasma separation membrane for immediate and non-destructive separation and extraction of plasma, and for extracting fresh blood serum. ADVANTAGE - The method enables preparation of biamino chitosan-graphene oxide hybrid material having rapid water molecular channel of graphene and biocompatibility and hydrophilicity of chitosan. DETAILED DESCRIPTION - Preparation of biamino chitosan-graphene oxide hybrid material involves (1) dissolving O-carboxymethyl chitosan in 2-(N-morpholine)ethanesulfonic acid (MES) buffer solution of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide/N-hydroxysuccinimide (EDC/NHS) to obtain solution (A), and stirring to react in an ice bath, (2) mixing the reaction solution obtained in the step (1) with the MES buffer solution of mono-tert-butoxycarbonyl-protected diamine to obtain solution (B), adding sodium hydroxide to control the pH to 6-8, and stirring the reaction at room temperature, (3) adding hydrochloric acid to adjust the pH of the solution to 4.9-5.1, filtering and washing to obtain a mono-tert-butoxycarbonyl protected diamino chitosan derivative, (4) dispersing the mono-tert-butoxycarbonyl-protected diamino chitosan derivative in methanol, slowly dripping concentrated hydrochloric acid, stirring the reaction, then dialyzing in deionized water, freeze-drying, and obtaining diamino chitosan derivative, (5) mixing a graphene oxide butyl acetate suspension with an condensing agent, and ultrasonically making it uniformly mixed to obtain a mixed solution (C), and (6) adding bisamino chitosan derivatives to the mixture (C), and stirring the reaction with temperature control, after completing the reaction, centrifuging the reaction solution, and washing with deionized water, ethanol and acetone cyclically, and then drying.