▎ 摘　　要

NOVELTY - Method for immobilizing enzyme for improving the stability of horseradish peroxidase, involves (a) preparing graphene oxide by performing modified Hummers chemical method, and (b) preparing zeolitic imidazolate framework-8/graphene complex material immobilized horseradish peroxidase HRP-ZIF-8/GO by ultrasonically mixing graphite and zinc nitrate in water to form a mixture A, dissolving horseradish peroxidase and 2-methylimidazole in water to form a mixture B, quickly pouring the mixture B into the mixture A, and stirring the resulting mixture at room temperature for 12-24 hours, washing the obtained mixture with high-purity water for 3-5 times using centrifugation, and lyophilizing for 12-24 hours to obtain a powdery substance, which was ground and stored at -4 degrees C to -20 degrees C. USE - The method is useful for immobilizing enzyme for improving the stability of horseradish peroxidase, which is useful for detecting kanamycin. ADVANTAGE - The method detects kanamycin in a specific manner. DETAILED DESCRIPTION - Method for immobilizing enzyme for improving the stability of horseradish peroxidase, involves (a) preparing graphene oxide by performing modified Hummers chemical method, and (b) preparing zeolitic imidazolate framework-8/graphene complex material immobilized horseradish peroxidase HRP-ZIF-8/GO by ultrasonically mixing graphite and zinc nitrate in water to form a mixture A, dissolving horseradish peroxidase and 2-methylimidazole in water to form a mixture B, quickly pouring the mixture B into the mixture A, and stirring the resulting mixture at room temperature for 12-24 hours, where the mass ratio of graphite to horseradish peroxidase is 0:50-1:1, and the ratio of horseradish peroxidase mass, mole of zinc nitrate and mole of 2-methylimidazole is 5 g:100 mol:7000 mol, washing the obtained mixture with high-purity water for 3-5 times using centrifugation, and lyophilizing for 12-24 hours to obtain a powdery substance, which was ground and stored at -4 degrees C to -20 degrees C. The product of the different GO content obtained was named HRP-ZIF-8/GO-x, where x represents the mass percentage of GO added in the final product. An INDEPENDENT CLAIM is included for use of an immobilized enzyme for increasing the stability of horseradish peroxidase by performing quantitative detection of kanamycin, which involves adding 0-50 mu g/l kanamycin solution and kanamycin aptamer solution in 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer at a temperature of 25-35 degrees C for 20-30 minutes, where, the concentration of HEPES buffer is 10-25 mM having a pH of 7-8, subsequently adding HRP-ZIF-8/GO-x complex to the mixture, incubating at 25-35 degrees C for 20-30 minutes, where the mass ratio of the HRP-ZIF-8/GO-x complex to the KAN aptamer is 4x 106 g:214 mol, adding KAN aptamers, 3,3',5,5'-tetramethylbenzidine (TMB) and hydrogen peroxide (H2O2)in a molar ratio of 214:50:11, reacting the mixture at 25-37 degrees C for 10-30 minutes, transferring to a quartz cuvette, and recording the absorbance of the system as a function of absorption wavelength, where the aptamer sequence is 5'-tgggggttgaggctaagccga-3 (SEQ ID NO: Not Defined).