▎ 摘　　要

NOVELTY - A DNA base detection method with electrochemical sensors involves mixing graphene oxide in double distilled water, subjecting product to ultrasonic dispersion, and dispersing product to obtain bromocresol purple solution. The product is added dropwise to glassy surface of carbon electrode. The modified grapheme electrodes are placed in bromocresol purple solution. The product is placed in detection base solution to determine electrochemical signals, followed by establishing electrochemical signal variation diagram. USE - Method for detecting DNA bases with electrochemical sensors. DETAILED DESCRIPTION - A DNA base detection method with electrochemical sensors involves mixing 0.2-0.5 mg/ml graphene oxide in double distilled water, subjecting product to ultrasonic dispersion for 10-15 minutes, and dispersing product in 0.01 M sodium nitrate solution to obtain bromocresol purple solution having concentration of 1asterisk103- 2asterisk103 M. The 5-10 mu l of product is added dropwise to glassy surface of carbon electrode. The product is placed under infrared lamp, and cooled to room temperature after drying to obtain glassy carbon electrode modified graphene oxide. The electrode is placed in phosphate buffer saline having pH of 5-7 under reduction potential of 1-1.5 V for 360-720 seconds and maintained at room temperature. The modified graphene electrodes are placed in bromocresol purple solution and then subjected to cyclic voltammetry with potential of 1-1.5 V, at scan rate of 50-100 mV/s for 10-15 cycles to obtain bromocresol purple/graphene composite modified electrode. The product is placed in detection base solution to determine electrochemical signals, where the working condition include potential increment of 0.005-0.015 V, base solution comprises 0.20-200.0 mu M guanine, 0.20-220 mu M adenine, 2-700 mu M thymidine, 2.50-650 mu M cytosine and 0.1-0.2 mu M phosphate buffer solution and pH of the base solution is 7-7.4, followed by establishing electrochemical signal variation diagram at temperature of 22-25 degrees C under pulse of 0.01-0.1 V, pulse width of 0.01-0.1 s, and potential increments of 0.005-0.015V.