• 专利标题:   Sample testing chip useful for detecting test target, comprises first layer formed of porous hydrophilic material, and hydrophobic barriers located in first layer to define testing areas configured to receive volume of sample.
  • 专利号:   WO2022060870-A1
  • 发明人:   WANG R, ERICKSON D
  • 专利权人:   UNIV CORNELL
  • 国际专利分类:   B01L003/00
  • 专利详细信息:   WO2022060870-A1 24 Mar 2022 B01L-003/00 202230 Pages: 44 English
  • 申请详细信息:   WO2022060870-A1 WOUS050510 15 Sep 2021
  • 优先权号:   US078377P

▎ 摘  要

NOVELTY - Sample testing chip (100) comprises (a) a first layer (102) formed of a porous hydrophilic material, and (b) one or more hydrophobic barriers (104) located in the first layer to define one or more testing areas configured to receive a volume of a sample and one or more auxiliary areas, where the one or more testing areas and the one or more auxiliary areas are separated from one another by the hydrophobic barrier and are not fluidically connected. USE - The sample testing chip is useful for detecting test target. The test target is (i) target microorganism, preferably pathogenic microorganism chosen from bacteria, Archaea or fungi, or (ii) target molecule chosen from a gene, DNA, RNA, an oligonucleotide or a polynucleotide, preferably a viral gene including severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) gene (all claimed). ADVANTAGE - The sample testing chip provides a user-friendly paper-based test that provides visual readout of test results, such as bacterial antibiotic susceptibility for example, in a semi- quantitative format. The chip utilizes on-chip paper microfluidics to enable multiplexed testing of multiple test samples with a single sample addition step, replicating the functionality of traditional broth-dilution-based susceptibility testing in a simplified format. It provides several advantages including low sample volume requirement and lack of need for humidity control during incubation. Eliminating the requirement of humidity control increases incubation flexibility. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is included for a method for detecting a test target. DESCRIPTION OF DRAWING(S) - The drawing shows top view of sample testing chip. Sample testing chip (100) First layer (102) Hydrophobic barrier (104) Central area (106) Testing area (108)