▎ 摘　　要

NOVELTY - Molecularly imprinted electrochemical sensor with double-signal probe strategy, which is prepared by fixing the first template molecule immunoglobulins (IgG) on the Prussian blue doped gold nano particle/graphene oxide/screen printing electrode (PB doped AuNPs/GO/SPCE) through gold sulfur bond to obtain the IgG/PB doped AuNPs/GO/SPCE, fixing the second template molecule immunoglobulin M (IgM) to the multi-wall carbon nano tube-thionine-gold nano particle/bovine serum albumin/IgG/Prussian blue doped gold nano particle/graphene oxide/graphene oxide/screen printing electrode (MWCNTs-TH-AuNPs/BSA/IgG/ PB doped AuNPs/GO/SPCE) to obtain IgM/MWCNTs-THAuNPs/BSA/IgG/PB doped AuNPs/GO/SPCE, performing electrochemical aggregate to obtain the pancreatic polypeptide/IgM/MWCNTs-THAuNPs/BSA/IgG/PB doped AuNPs/GO/SPCE, eluting IgG and IgM with eluent to form three-dimensionally complementary template molecules and imprinting cavities to obtain molecularly imprinted electrochemical sensors. USE - The sensor is useful for detecting IgG and IgM (claimed). ADVANTAGE - The sensor has high sensitivity, good selectivity, fast analysis speed and easy miniaturization.