▎ 摘　　要

NOVELTY - Preparation of biosensor electrode involves ultrasonically cleaning indium-tin oxide conductive glass with acetone solution, ethanol solution and deionized water for 20 minutes, naturally cooling, drying, fixing resultant product in a spraying equipment heating plate, such that the glass and electrode line are in direct contact, spraying an uniform suspension prepared using suspension of molybdenum disulfide nanoparticles and foamed graphene on resultant product by automatic spraying method using a spray gun, heating, and cooling to room temperature. USE - Preparation of biosensor electrode used for detection of levodopa. ADVANTAGE - The method enables preparation of biosensor electrode having excellent electroconductivity. The electrode enables efficient detection of levodopa with high sensitivity. DETAILED DESCRIPTION - Preparation of biosensor electrode involves ultrasonically cleaning indium-tin oxide conductive glass with acetone solution, ethanol solution and deionized water for 20 minutes, naturally cooling, drying, fixing resultant product in a spraying equipment heating plate, such that the glass and electrode line are in direct contact, spraying an uniform suspension on resultant product by automatic spraying method using a spray gun at inlet pressure of 10 psi, distance from nozzle to the conductive glass of 15 cm, and heating plate temperature of 110 degrees C, placing resultant product in a quartz tube furnace in 400 sccm argon atmosphere, heating to 700 degrees C at 10 degrees C/minute for 2 hours, and cooling to room temperature. The uniform suspension is prepared by adding foamed graphene to deionized water, ultrasonically processing using ultrasonic processor at 540 W for 1-4 hours, obtaining graphene suspension, mixing 0.5 mL suspension of molybdenum disulfide nanoparticles with 19.5 mL graphene suspension in a ratio of 9:1, obtaining mixed solution of molybdenum disulfide-graphene composite nanosheets, and ultrasonically processing. The foamed graphene is prepared by adding foamed nickel to quartz tube furnace, heating to 1000-1100 degrees C at 20-40 degrees C/minute in argon and hydrogen atmosphere, heat-preserving for 30-60 minutes, introducing methane gas at 5-10 sccm for 5-20 minutes, cooling to room temperature at 80-100 degrees C/minute, obtaining graphene-coated foamed nickel having foam nickel density of 420-440 g/m2 and thickness of 1.6-2 mm, adding poly(methyl methacrylate) to ethyl lactate, heating, stirring at 80-120 degrees C for 1-2 hours, obtaining mixed solution, adding the mixed solution to graphene-coated foamed nickel, drying at 150-200 degrees C, heat-preserving for 0.5-1 hour, obtaining poly(methyl methacrylate)-coated graphene, cutting resultant product into a cube having a surface area of 0.5-2 cm2, immersing in 3-4 mol/L hydrochloric acid solution at 80-90 degrees C for 4-6 hours, immersing resultant nickel-removed three-dimensional foamed graphene in acetone at 60-70 degrees C for 0.5-1.5 hours, washing with distilled water to remove poly(methyl methacrylate), and drying. The suspension of molybdenum disulfide nanoparticles is prepared by adding 30 mg/mL molybdenum disulfide powder to mixed solution of acetone and water in a volume ratio of 89:11, ultrasonically processing using ultrasonic processor at 540 W for 1-4 hours, centrifuging at 3000 rpm for 30 minutes, drying in a blast oven, dispersing in deionized water, and obtaining suspension of molybdenum disulfide nanoparticles with concentration of 20 mg/mL. The flow rate of the argon gas is 480-500 sccm. The flow rate of hydrogen is 180-200 sccm. The content of methyl methacrylate in the mixed solution is 4-5 %mass.