▎ 摘　　要

NOVELTY - Electrochemical luminescence detection of spermidine involves dispersing worm graphite in ammonium sulfate solution to obtain graphite dispersion, using platinum electrodes, performing electrolysis for 24 hours, filtering, washing with water and ethanol, dialyzing for 3 days, placing in a freeze dryer to obtain graphene powder, taking graphene powder, dispersing in water/ethanol mixture to obtain graphene dispersion, ultrasonically dispersing the graphene dispersion in Nafion (RTM: Perfluorosulfonic acid-PTFE), taking Nafion (RTM: Perfluorosulfonic acid-PTFE)-graphene dispersion on the surface of the glassy carbon electrode, immersing the electrode in tris(bipyridine)ruthenium(II) chloride solution, washing with deionized water, leaving, drying, forming graphene surface with tris(bipyridine)ruthenium(II) chloride functional group and preparing solid-state electrochemical light-emitting sensor with tris(bipyridine)ruthenium(II) chloride/graphene-modified electrode. USE - The method is useful for electrochemical luminescence detection of spermidine. ADVANTAGE - The method enables simple electrochemical luminescence detection of spermidine with high sensitivity, specific surface area and recovery rate of 96-103% and excellent electrical conductivity and electrocatalytic activity in environmentally-friendly manner. DETAILED DESCRIPTION - Electrochemical luminescence detection of spermidine involves dispersing worm graphite in 1 M ammonium sulfate solution through a shearing machine to obtain graphite dispersion, using electrode electrochemical system to exfoliate the dispersion, using platinum electrodes as working and counter electrodes, applying constant voltage of 10 V using direct current power supply, performing electrolysis for 24 hours to obtain stripped graphene solution, filtering, washing with water and ethanol, dialyzing for 3 days, placing in a freeze dryer for 48 hours to obtain graphene powder, taking 1 mg graphene powder, dispersing in 1 ml water/ethanol mixture to obtain 1 mg/ml graphene dispersion, ultrasonically dispersing the graphene dispersion in 1 wt.% Nafion (RTM: Perfluorosulfonic acid-PTFE) to prepare uniform Nafion (RTM: Perfluorosulfonic acid-PTFE)-graphene dispersion, taking 10 mu l Nafion (RTM: Perfluorosulfonic acid-PTFE)-graphene dispersion on the surface of the glassy carbon electrode, drying at room temperature, immersing the electrode in 10 mM tris(bipyridine)ruthenium(II) chloride solution for 1 hour, taking the electrode, washing with deionized water, leaving, drying, adsorbing cation exchanger Nafion (RTM: Perfluorosulfonic acid-PTFE), fixing tris(bipyridine)ruthenium(II) chloride through cation exchange, forming graphene surface with tris(bipyridine)ruthenium(II) chloride functional group and preparing solid-state electrochemical light-emitting sensor with tris(bipyridine)ruthenium(II) chloride/graphene-modified electrode.