• 专利标题:   Preparing antibody-modified hydrophilic graphene film material used for capturing e.g. prostate cancer cell DU145, by fixing graphene film on adhesive substrate, coating chitosan layer, grafting polyamidoamine and conjugating antibody.
  • 专利号:   CN106801040-A
  • 发明人:   GAO M, ZHANG P, ZHANG X
  • 专利权人:   UNIV FUDAN
  • 国际专利分类:   C01B032/194, C12N005/09
  • 专利详细信息:   CN106801040-A 06 Jun 2017 C12N-005/09 201757 Pages: 8 Chinese
  • 申请详细信息:   CN106801040-A CN10062302 02 Feb 2017
  • 优先权号:   CN10062302

▎ 摘  要

NOVELTY - Method for preparing antibody-modified hydrophilic graphene film material involves uniformly fixing graphene thin film on double-sided adhesive substrate, coating chitosan layer by coupling reaction, grafting polyamidoamine (PAMAM) dendrimer molecules to chitosan through glutaraldehyde crosslinking method, covalently conjugating modified antibody on surface of the graphene film material using carbodiimide and obtaining thin film material having immune recognition and capture function. USE - The method is useful for preparing antibody-modified hydrophilic graphene film material, which is used for capturing prostate cancer cell DU145 and human cervical cancer cell Hela, and antigens corresponding to antibodies on tumor cell membrane (all claimed). ADVANTAGE - The method exhibits 85 plus minus 3% tumor cell capture efficiency within 45 minutes. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is included for use of antibody-modified hydrophilic graphene film material for capturing prostate cancer cell DU145 and human cervical cancer cell Hela.