▎ 摘　　要

NOVELTY - In-situ quantitative analysis of heterocyclic polycyclic aromatic hydrocarbon adsorbed on plant root surface involves washing root of model plant, drying, immersing the root in a graphene quantum dot solution, selecting a specific plant root region, applying a heterocyclic polycyclic aromatic hydrocarbon solution to the epidermis, performing fluorescence spectroscopy, obtaining a fluorescence quenching constant, establishing a standard curve of the fluorescence quenching constant and the concentration of the heterocyclic polycyclic aromatic hydrocarbon adsorbed on the root epidermis of the plant, washing to-be tested roots of the plants, drying, immersing washed roots in graphene quantum dot solution, selecting a specific plant root region, performing fluorescence spectroscopy, obtaining fluorescence quenching constant, and obtaining the concentration of heterocyclic polycyclic aromatic hydrocarbon adsorbed to the root epidermis of the plant based on the standard curve. USE - In-situ quantitative analysis of heterocyclic polycyclic aromatic hydrocarbon adsorbed on plant root surface by fluorescence quenching method based on graphene quantum dot. ADVANTAGE - The concentration of heterocyclic polycyclic aromatic hydrocarbon adsorbed on plant root surface is analyzed with high sensitivity. The quantitative analysis method effectively prevents the photo damage caused by ultraviolet light of laser-induced nanosecond time-resolving method.