▎ 摘　　要

NOVELTY - Protein adsorption material, prepared by taking graphene oxide as the carrier, introducing excessive hydrogen-containing polyethyleneimine (PEI) containing amino groups on the surface of the graphene oxide carrier through the interaction of hydrogen bonds and static electricity, carrying out redox reaction with chloroauric acid through amino groups to obtain nano-gold particles to achieve the modification of nano-gold on the surface of the material, and coating the material on the surface of the polymer microsphere through a non-covalent bonding method to prepare a protein adsorption material containing more fixed gold nano particles. USE - The protein adsorption material is useful for processing proteins at least one of samples in cells, tissues, plasma or urine (all claimed). ADVANTAGE - The material: preparation method can utilize the physical adsorption of nano-gold and protein, non-covalent adsorption, and chemical covalent binding to achieve selective adsorption of protein, has simple material synthesis and provides easy operation with high protein recovery rate and strong anti-interference ability. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is also included for application of protein adsorption material, comprising (i) denaturing amino polymer ball-graphene oxide-polyethyleneimine-gold (PM-GO/PEI/Au) when processing samples, reducing the protein sample to fully expose the sulfhydryl groups in the protein, adding it at room temperature, adding PM-GO/PEI/Au material, where the quality of the material is the amount of protein processed 5-100 times, reacting at 15-40 degrees C for 0.5-4 hours, shaking at 1000-1500 revolutions/minute to ensure that the material is fully combined with the sample, centrifuging at 5000-16000 g and removing the supernatant; (ii) washing with deionized water or 30-100% methanol solution for 2-5 times, utilizing a phosphate buffer solution (PB) with a concentration of 25-75mM pH 7.5-8.5 or washing with a pH of 7.5-8.5 (25-75 mM) ammonium bicarbonate solution for 2-5 times to make the supernatant clear and colorless, dissolving the obtained solid phase of the adsorbed protein sample in 25-75mM ammonium bicarbonate solution with a pH of 7.5-8.5, adding trypsin according to the ratio of enzyme to protein mass ratio of 1:25-1:100, reacting fully at 30-40 degrees C, digesting the protein from the surface of the material into the solution, centrifuging supernatant, lyophilizing, reconstituting and subjecting directly to mass spectrometry analysis.