▎ 摘　　要

NOVELTY - The nanosensor (100) has a substrate (10) which includes a hole that extends through the substrate. A thin layer (20) on the substrate includes nano-pore (25) that is connected to the hole. A right graphene (31) layer and left graphene layer (33) on the thin layer are spaced apart from each other and centering between the nano-pore. The thin layer includes an oxide or a nitride and one selected from the group consisting of silicon nitride (SiN), silicon dioxide (SiO2), aluminum trioxide (Al2O3), titanium dioxide (TiO2), barium titanate (BaTiO3), and lead titanate (PbTiO3). USE - Nanosensor for detecting nucleic acid and for detecting and sequencing DNA using nano-pores. ADVANTAGE - The graphene layers of the nanosensor have thickness of about 0.34 nm, thus better electric conductivity can be achieved and the nanosensor is capable of determining order of DNA bases quickly and accurately, without cutting single-stranded DNA molecule randomly and thus costs can also be reduced. By using insulating layer, the graphene layers from outer elements can be protected and the parasitic current can be prevented. Since the electrical signal of nano gap is provided between the graphene layers, the noise from outside elements can be reduced or effectively prevented and thus target molecules are distinguished more accurately. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is included for method of manufacturing nanosensor. DESCRIPTION OF DRAWING(S) - The drawing shows a plan view of nanosensor. Substrate (10) Thin layer (20) Nano pore (25) Right graphene layer (31) Left graphene layer (33) Nanosensor (100)