▎ 摘　　要

NOVELTY - Use of graphene nanoplatelets reinforced chitosan scaffolds for intracellular delivery, is claimed. USE - Graphene nanoplatelets reinforced chitosan scaffolds is used for intracellular delivery of biomolecules, such as drug delivery, dye delivery and genetic material delivery under the exposure of nanosecond pulsed laser mediated optoporation, and is used for laser mediated delivery along with genetic material delivery (all claimed). ADVANTAGE - The graphene nanoplatelets reinforced chitosan scaffolds offers regeneration of tissues with no toxicity, which shows high tissue regeneration mimicking the native functional tissues. The graphene nanoplatelets reinforced chitosan scaffolds for intracellular delivery of biomolecules enables to carry out drug delivery, dye delivery and genetic material delivery under the exposure of nanosecond pulsed laser mediated optoporation. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for: (1) a method for preparing graphene nanoplatelets reinforced chitosan scaffolds, which involves: (a) dissolving chitosan in acetic acid solution to form a chitosan solution; (b) dispersing graphene nanoplatelets in acetic acid solution via probe sonication; (c) mixing chitosan solution in graphene nanoplatelets solution, carrying out magnetic stirring at a temperature in the range of 60-80℃ to form a homogeneous solution; and (d) filling homogeneous solution in a glass casting box and drying in hot air oven at a temperature in the range of 70-90℃ for overnight to produce a carbon graphene nanoplatelets reinforced chitosan scaffolds in the form of a film; and (2) a method for performing the intracellular delivery of biomolecules via nanosecond laser pulse mediated optoporation using graphene nanoplatelets reinforced chitosan scaffolds, which involves: (a) culturing of cells on the graphene nanoplatelets reinforced chitosan scaffolds to facilitate optoporation; and (b) carrying out nanosecond pulsed laser assisted delivery to allow biomolecules to enter from outside, where cells remain viable after delivery of biomolecules.