▎ 摘　　要

NOVELTY - Targeting and removing drug resistance of bacteria comprises assembling the nucleic acid molecule of the targeted antibiotic resistance gene with the nano-carrier with sharp edge, obtaining the targeting complex, where the nucleic acid molecule comprises a first sequence encoding a clustered regularly interspaced short palindromic repeats (CRISPR) related nuclease and a second sequence generating sgRNA targeting the antibiotic resistance gene, contacting the targeting complex with the bacteria, delivering the nucleic acid molecules into the bacteria through the physical puncture action of the nano-carrier and knocking out the antibiotic resistance gene of the bacteria, where the sharp edge meets the requirement that the edge of the nanocarrier has 1-100 outward atomic layers. USE - The method is useful for targeting and removing drug resistance of bacteria. ADVANTAGE - The method: uses nanoparticles with sharp edges as carriers to physically puncture the bacterial cell wall and cell membrane by considering that the rigidity of the bacterial cell wall is weaker than that of the plant cell wall; realizes the transmembrane transport of exogenous nucleic acid molecules on bacteria, to realize the delivery of exogenous CRISPR system, and present Antimicrobial resistance genes (ARG)-CRISPR in this way to target and eliminate bacterial drug resistance. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are also included for: a target complex for removing drug resistance of bacteria; and preparing the targeting complex. DESCRIPTION OF DRAWING(S) - The drawing shows a schematic flow diagram of a method for targeting and removing drug resistance of bacteria. (Drawing includes non-English language text).