▎ 摘　　要

NOVELTY - Biosensor for detecting target DNA comprises a gold nanosubstrate having a flower-shaped gold nanostructure introduced to it and having graphene oxide immobilized on it; a gold nanoparticle complex in which gold nanoparticles, a Raman marker, and single-stranded DNA to be analyzed are linked; and CRISPR RNA (crRNA) and Cas12a endonuclease for recognizing the target DNA. USE - The biosensor is useful for detecting target DNA (claimed). ADVANTAGE - The biosensor for detecting target DNA is manufactured using a gold nanoparticle complex having surface-enhanced Raman scattering activity and a gold nano substrate, and thus has excellent sensitivity and stability of Raman signals. By using the biosensor, target DNA can be quantitatively detected at a femtomolar concentration level in a simple and economical way. The biosensor provides a high-sensitivity platform that can measure target DNA quickly and accurately, and can be used as a real-time measurement and body fluid-based on-site disease diagnosis kit. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is included for detecting target DNA which involves preparing a gold nanoparticle complex by reacting gold nanoparticles, a Raman marker, and single-stranded DNA to be analyzed; fixing the gold nanoparticle complex to a gold nanosubstrate having a flower-shaped gold nanostructure introduced to it and having graphene oxide fixed on it; reacting the gold nanosubstrate on which the gold nanoparticle complex is immobilized with crRNA and Cas12a endonuclease for recognizing target DNA; and measuring a surface-enhanced Raman scattering (SERS) signal.