▎ 摘　　要

NOVELTY - Composition comprises locked nucleic acid (LNA)-containing molecular beacon; and graphene oxide. The molecular beacon is formed by complementarily conjugating the first single strand containing the target nucleic acid binding sequence and the second single strand bound with graphene oxide at least in portion; and the first single strand is separated from the second single strand and bound to the target nucleic acid in the presence of the target nucleic acid. USE - The composition or kit is useful for detecting a nucleic acid (claimed). ADVANTAGE - The molecular beacon and graphene oxide can be covalently bonded to minimize non-specific signals, and a LNA-added molecular beacon is designed in a double strand to detect a very low concentration of target nucleic acid with high sensitivity, as well as a fluorescent signal, and the multiple target nucleic acids can be detected simultaneously through diversification of the fluorescent signal to enable easy and accurate detection of a nucleic acid biomarker whose specific expression level is specifically changed according to diseases and disease progression. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for: (1) detecting a nucleic acid which involves mixing a composition for detecting nucleic acid and a sample containing a target nucleic acid; and detecting the target nucleic acid, where, the composition for detecting nucleic acid is prepared by: conjugating complimentarily a first single strand containing a target nucleic acid binding sequence with a second single strand containing a functional group to react with graphene oxide to prepare a molecular beacon, where the target nucleic acid binding sequence contains at least one LNA; reacting the molecular beacon with graphene oxide (GO) to prepare GO-LNA-containing molecular beacon v bound with graphene oxide at least in portion; and (2) a kit for detecting nucleic acid containing a composition comprising LNA-containing molecular beacon and graphene oxide, where the molecular beacon is formed by complementarily conjugating the first single strand containing the target nucleic acid binding sequence and the second single strand bound with graphene oxide at least in portion, and the first single strand is separated from the second single strand and bound to the target nucleic acid in the presence of the target nucleic acid.