▎ 摘 要
NOVELTY - An electrochemical aptasensor comprises a working electrode which is surface-modified with graphene nanoplatelet composite including graphene nanoplatelets, transition metal chalcogen compound, and amine-based polymer, gold nanoflowers bound to an upper end of the working electrode, and an aptamer binding to a target material and is immobilized on the gold nanoflowers. USE - Electrochemical aptasensor for analyzing target material e.g. di(2-ethylhexyl)phthalate (all claimed), and preventing health problems from exposure to trace amount of di(2-ethylhexyl)phthalate. ADVANTAGE - The electrochemical aptasensor has low detection limit concentration by improving sensitivity by sensor surface modification using nano composite and gold nanoflowers, and detects target material with high sensitivity, in simple measurement method. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for: 1. A method of producing an electrochemical aptasensor, which involves: (a) depositing graphene nanoplatelet composite including graphene nanoplatelets, transition metal chalcogen compound, and an amine-based polymer on a working electrode; (b) electrodepositing gold precursor on the working electrode to produce gold nanoflowers; and (c) immobilizing an aptamer capable of binding to a target material on the gold nanoflowers; and 2. A method of analyzing a target material, which involves: (a) preparing a sample for analysis; (b) injecting the prepared sample into the electrochemical aptasensor; (c) forming a composite by the target material specifically binding to an aptamer immobilized on a working electrode; and (d) measuring an electrochemical signal produced by the composite to analyze the target material in the sample for analysis.