• 专利标题:   Electrochemical aptasensor for analyzing target material, comprises working electrode modified with graphene nanoplatelet composite including graphene nanoplatelets, transition metal chalcogen compound and amine-based polymer, gold nanoflowers bound to electrode, and aptamer bound to target material.
  • 专利号:   US2023142624-A1, KR2023068690-A
  • 发明人:   LEE K, JUNG H, LEE K Y
  • 专利权人:   UNIV YONSEI IND ACADEMIC COOP FOUND, UNIV YONSEI IND ACADEMIC COOP FOUND
  • 国际专利分类:   C25D003/48, C25D005/48, C25D007/00, G01N027/30, G01N027/327, G01N033/53, G01N033/543, B82Y015/00, B82Y030/00, G01N033/44
  • 专利详细信息:   US2023142624-A1 11 May 2023 G01N-027/327 202345 English
  • 申请详细信息:   US2023142624-A1 US054340 10 Nov 2022
  • 优先权号:   KR154747

▎ 摘  要

NOVELTY - An electrochemical aptasensor comprises a working electrode which is surface-modified with graphene nanoplatelet composite including graphene nanoplatelets, transition metal chalcogen compound, and amine-based polymer, gold nanoflowers bound to an upper end of the working electrode, and an aptamer binding to a target material and is immobilized on the gold nanoflowers. USE - Electrochemical aptasensor for analyzing target material e.g. di(2-ethylhexyl)phthalate (all claimed), and preventing health problems from exposure to trace amount of di(2-ethylhexyl)phthalate. ADVANTAGE - The electrochemical aptasensor has low detection limit concentration by improving sensitivity by sensor surface modification using nano composite and gold nanoflowers, and detects target material with high sensitivity, in simple measurement method. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for: 1. A method of producing an electrochemical aptasensor, which involves: (a) depositing graphene nanoplatelet composite including graphene nanoplatelets, transition metal chalcogen compound, and an amine-based polymer on a working electrode; (b) electrodepositing gold precursor on the working electrode to produce gold nanoflowers; and (c) immobilizing an aptamer capable of binding to a target material on the gold nanoflowers; and 2. A method of analyzing a target material, which involves: (a) preparing a sample for analysis; (b) injecting the prepared sample into the electrochemical aptasensor; (c) forming a composite by the target material specifically binding to an aptamer immobilized on a working electrode; and (d) measuring an electrochemical signal produced by the composite to analyze the target material in the sample for analysis.