▎ 摘　　要

NOVELTY - Biosensor comprises a first non-conducting substrate comprising a primary substrate surface, a conducting electrode layer comprising one or more conducting sub-layers including a first electrode sub-layer and a second electrode sub-layer, where the conducting electrode layer comprises a primary electrode surface and a secondary electrode surface, where the secondary electrode surface covers portion of the primary substrate surface, a probe layer bonded to portion of the primary electrode surface, and the probe layer being adapted for selectively binding of the target substance, and a second non-conducting substrate comprising a secondary substrate surface, where the secondary substrate surface of the second substrate and the primary substrate surface of the first substrate are interconnected such that the electrode layer and the probe layer are confined within an area defined by the first substrate and the second substrate. USE - The biosensor is useful for detecting target substance in a sample with impedance spectroscopy, for point-of-care measurement and/or on-site detecting of a target substance in a liquid sample such as e.g. water, blood, urine, saliva, food product or a sample from a test surface or for point-of-care measurement and/or on-site detecting of a target substance in a liquid sample obtained during process and/or quality control measurements, during manufacturing of medicine, during manufacture of agents for therapy, or during a content control process in connection with food preparation. The target substance is an antibody (all claimed). ADVANTAGE - The biosensor is cost-effective and easily mass-produced and has high integration, low sample- and reagent volume, short analysis time, and low sample waste. DETAILED DESCRIPTION - Biosensor comprises (1) a first non-conducting substrate comprising a primary substrate surface, a conducting electrode layer comprising one or more conducting sub-layers including a first electrode sub-layer and a second electrode sub-layer, where the conducting electrode layer comprises a primary electrode surface and a secondary electrode surface, where the secondary electrode surface covers portion of the primary substrate surface, a probe layer bonded to portion of the primary electrode surface, and the probe layer being adapted for selectively binding of the target substance, and a second non-conducting substrate comprising a secondary substrate surface, where the secondary substrate surface of the second substrate and the primary substrate surface of the first substrate are interconnected such that the electrode layer and the probe layer are confined within an area defined by the first substrate and the second substrate, where the electrode layer comprises at least a first electrode pair with a primary electrode and a secondary electrode, where the probe layer is bonded to the primary electrode and/or the secondary electrode, where the first electrode sub-layer is a conducting polymer electrode layer, and the second electrode sub-layer is positioned between the first electrode sub-layer and the probe layer, and is chosen from a redox material layer, a polymerized redox material electrode layer, a functionalization layer, a polymerized functionalization layer, a graphene oxide layer o a modified graphene oxide layer, or a nanoparticle layer or (2) a first non-conducting substrate comprising a primary substrate surface, a conducting electrode layer comprising one or more electrode sub-layers including a first electrode sub-layer, where the electrode layer comprises a primary electrode surface and a secondary electrode surface, where the secondary electrode surface covers portion of the primary substrate surface, a probe layer bonded to portion of the primary electrode surface, and the probe layer being adapted for selectively binding of the target substance, and a second non-conducting substrate comprising a secondary substrate surface, where the secondary substrate surface of the second substrate and the primary substrate surface of the first substrate are interconnected such that the electrode layer and the probe layer are confined within an area defined by the first substrate and the second substrate, where the electrode layer comprises at least a first electrode pair with a primary electrode and a secondary electrode, where the probe layer is bonded to the primary electrode and/or the secondary electrode, where the first electrode sub-layer is chosen from a carbon electrode layer, a glassy carbon electrode layer, a graphene electrode layer, a modified graphene oxide layer, a two-dimensional transition-metal dichalcogenide layer, a hexagonal boron nitride layer, a graphene electrode layer comprising a redox material, a conducting polymer electrode layer comprising a redox material, a conductive polymer electrode layer comprising nanoparticles, a conductive polymer electrode layer comprising two-dimensional transition-metal dichalcogenide, or a conductive polymer electrode layer comprising hexagonal boron nitride. An INDEPENDENT CLAIM is included for a system for detecting target substance in a sample.