▎ 摘　　要

NOVELTY - Optogenetic neural repair scaffold compounded with upconverting nanoparticle (UCNP) comprises a core-shell nanoparticle (sodium yttrium fluoride (NaYF4):ytterbium (Yb):Tm-NaYF4), where shell layer is a thin layer of sodium tetrafluoroyttrium and core is sodium tetrafluoroyttrium doped with ytterbium/terbium, and outermost layer is a thin silicon dioxide (SiO2) layer with good tissue compatibility in vivo (UCNP-SiO2 for short). The scaffold comprises (i) electrospun parallel filament membrane compounded with UCNP-SiO2 UCNP, and (ii) chitosan-graphene oxide (CS-GO) film directly compounded with UCNP-SiO2, and rolled into a tubular scaffold for nerve repair. USE - The optogenetic neural repair scaffold is useful for repairing and treating brachial plexus injury, common peroneal nerve injury, lateral femoral cutaneous neuritis, sciatica, intercostal nerve, ulnar nerve injury, radial nerve injury, median nerve injury, tibial nerve and other peripheral nerve injury, and spinal cord injury (all claimed). ADVANTAGE - The optogenetic neural repair scaffold has excellent biocompatibility and degradability, and promotes regeneration of nerve damage. DETAILED DESCRIPTION - Optogenetic neural repair scaffold compounded with upconverting nanoparticle (UCNP) comprises a core-shell nanoparticle (sodium yttrium fluoride (NaYF4):ytterbium (Yb):Tm-NaYF4), where shell layer is a thin layer of sodium tetrafluoroyttrium and core is sodium tetrafluoroyttrium doped with ytterbium/terbium, and outermost layer is a thin silicon dioxide (SiO2) layer with good tissue compatibility in vivo (UCNP-SiO2 for short). The scaffold comprises (i) electrospun parallel filament membrane compounded with UCNP-SiO2 UCNP, and (ii) chitosan-graphene oxide (CS-GO) film directly compounded with UCNP-SiO2, and rolled into a tubular scaffold for nerve repair. The nerve is repaired by bridging or placing scaffold on nerve injury with surgical sutures, injecting virus carrying gene for rhodopsin (a cation channel protein i.e. ChR2) into both ends of the inside of the tube, allowing the virus to transfect ChR2 in Schwann cells and nerve axons at the site of nerve injury genes, expressing ChR2 protein on their cell membrane, where UCNP-SiO2 on the scaffold absorbs near-infrared light and converts it to emit blue fluorescence, stimulating the ChR2 channel protein nearby to open, flowing extracellular cations into the cell, inducing and increasing the mitochondrial activity of the cells and axons where ChR2 is located, and promoting the growth of cells, the expression of neural factors, the extension of axons, and the repair of nerve damage. INDEPENDENT CLAIMS are included for the following: a method for preparing optogenetic neural repair scaffold compounded with UCNP involves (a) preparing UCNP particles coated with SiO2 by dispersing self-made UCNP in polyvinylpyrrolidone (PVP) solution (containing 18 ml ultrapure water and 900 mg PVP), ultrasonically processing for 20 minutes and stirring for 1 hour to prepare a UCNP dispersion, adding 80 ml methanol solution, ultrasonically processing for 20 minutes and stirring for 2 hours, adding 3.2 ml of 1-100 mM aqueous ammonia, ultrasonically processing for 20 minutes, adding 20-30 μl tetraethyl orthosilicate liquid and stirring for 30-60 minutes, washing precipitate separated by centrifugation 3 times with absolute ethanol, and dispersing in 4 ml ultrapure water to obtain UCNP-SiO2 with a SiO2 coating layer with a thickness of 5-100 nm, (b) electrospinning parallel filament membranes complexed with UCNP by soaking the self-made electrospun parallel silk membrane in 0.01-0.5 mg/ml poly-lysine solution for 5-60 minutes, taking it out and drying it naturally, soaking again, repeating 2-8 times to make the outermost layer with have a large amount of negative charge, soaking 5-20 mg/ml negatively charged electrospun parallel filament membrane in UCNP-SiO2 dispersion solution, stirring gently for 2-30 minutes, taking out to dry naturally, soaking and drying again, and repeating 2-8 times to obtain an electrospun parallel filament film compounded with UCNP, (c) preparing chitosan-graphene film complexed with upconverting nanoparticles by dissolving CS powder in 0.5-2 volume/volume% acetic acid solution to prepare 1-3 volume/volume% CS solution, performing ultrasonic dispersion of GO in 0.1-0.3 weight/volume% deionized water, ultrasonically mixing CS, UCNP-SiO2, and GO dispersion mixture at a mass ratio of 100:(30-3):(0.3-3), mixing 0.5-1.5 weight/volume% genipin solution to CS mass in a ratio of 0.3-3%, stirring at room temperature to 37°C for 2 hours and ultrasonically processing for 15 minutes, pouring the reaction solution on the template and drying at 37°C to obtain a film with a thickness of 0.5-2 mm, and (d) rolling nerve repair scaffolds by using polytetrafluoroethylene or high-density polyethylene thin sticks as templates, rolling electrospun parallel silk membrane compounded with UCNP into a tubular shape along the parallel method of the silk axis to improve its suture strength, spinning electrospun disordered silk film on the outermost layer for 3-4 layers, rolling and sewing the chitosan-graphene film compounded with UCNP into a tube shape, and obtaining nerve repair scaffold with a diameter of 0.1-5 mm and a length of 1-10 cm; and use of optogenetic neural repair scaffold compounded with UCNP for repairing nerve damage.