▎ 摘　　要

NOVELTY - Production of protein molecularly imprinted (MIP) electrochemical sensor involves surface-treating glassy carbon electrode, coating graphene aqueous solution on the electrode surface, drying, dispersing obtained graphene-modified glassy carbon electrode surface in a chitosan-copper solution, drying, immersing obtained product in template protein, functional monomer and crosslinking agent deoxygenated phosphate buffer, performing cyclic voltammetry, immersing resultant product in eluent, performing cyclic voltammetry, and obtaining working electrode. USE - Production of protein molecularly imprinted electrochemical sensor used for detecting lysozyme (all claimed). ADVANTAGE - The method enables production of protein molecularly imprinted electrochemical sensor with high sensitivity, and fast and high sensitivity template molecule selection recognizing ability. The lysozyme is rapidly detected using the molecularly imprinted electrochemical sensor. DETAILED DESCRIPTION - Production of protein molecularly imprinted (MIP) electrochemical sensor involves surface-treating glassy carbon electrode, coating graphene aqueous solution on the glassy carbon electrode surface, drying using an infrared lamp, obtaining graphene-modified glassy carbon electrode (GR/GCE), dispersing GR/GCE surface in a chitosan-copper (CS-Cu) solution, drying, obtaining Cu-CS/GR/GCE, immersing Cu-CS/GR/GCE in template protein which is 1 g/L lysozyme (Lyz), functional monomer which is 0.05-0.12 mol/L methacrylic acid and crosslinking agent deoxygenated phosphate buffer, performing cyclic voltammetry at voltage of -0.2 V to 1.2 V and speed of 100 mV/second, immersing obtained Lyz MIP-Cu-CS/GR/GCE in a predetermined concentration of eluent, performing cyclic voltammetry at voltage of -0.2 V to 1.2 V and speed of 100 mV/second and eluting lysozyme molecule, and obtaining molecularly imprinted electrochemically modified electrode (MIP-Cu-CS/GR/GCE) as a working electrode. The crosslinking agent is 0.01-0.2 mol/L 2-ethyl-4-methylimidazole. The protein molecularly imprinted electrochemical sensor comprises molecularly imprinted electrochemically modified electrode (MIP-Cu-CS/GR/GCE) as working electrode, a saturated calomel electrode as a reference electrode and a counter electrode. An INDEPENDENT CLAIM is included for application of protein molecularly imprinted (MIP) electrochemical sensor in detection of lysozyme.