▎ 摘　　要

NOVELTY - A graphene sensor device comprises a support substrate, a graphene layer deposited on the support substrate, a functionalization layer comprising solid state material deposited continuously on at least a portion of the graphene layer, and electrodes in electrical contact with the graphene layer and adapted to detect an electrical signal from the graphene layer. USE - Graphene sensor device used for detecting and quantifying concentration of ligand molecule and quantifying binding kinetics, preferably dissociation constant between macromolecule e.g. receptor and ligand (all claimed). Uses include but are not limited to proteins, peptides, nucleic acids, antibodies, hormones, toxins, neurotransmitters, nanoparticles, ions, or drugs. ADVANTAGE - The graphene sensor device has high sensitivity and selectivity, and effectively detects and quantifies ligand molecule concentrations in the samples. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for the following: (1) synthesis of graphene sensor device, which involves providing a graphene field effect transistor comprising a graphene layer and a pair of electrodes in electrical contact with the graphene layer, and forming a functionalization layer continuously on the graphene layer; (2) detection of ligand molecule, which involves (a) providing the device, (b) applying an electrical potential to the graphene layer, (c) contacting the device with a sample suspected of comprising a ligand molecule of interest, and (d) collecting information generated by the device comprising changes in electrical current over time to determine whether or not the ligand molecule is present in the sample; (3) quantification of concentration of ligand molecule, which involves (a) providing the device, (b) applying electrical potential to the graphene layer, (c) contacting the device with sample suspected of comprising ligand molecule of interest, and (d) collecting information generated by the device comprising the magnitude of change in electrical signal before and after contacting the device with the sample, and comparing the data to a database comprising change in electrical signal from known concentrations of the ligand molecule; and (4) quantification of binding kinetics between macromolecule and ligand, which involves (a) providing the device, (b) applying electrical potential to the graphene layer, (c) contacting the device with sample suspected of comprising ligand molecule of interest, and (d) collecting information generated by the device comprising changes in electrical current over time to quantify the binding kinetics, preferably dissociation constant between the macromolecule and the ligand.