▎ 摘　　要

NOVELTY - Method for preparing immunosensor involves dropping graphene oxide dispersion to the surface of the electrode substrate, drying, immersing in potassium chloride solution, scanning the electrode substrate using cyclic voltammetry, immersing the degraded graphene modified electrode in Prussian blue solution, scanning the electrode using cyclic voltammetry, dropping the Porcine epidemic diarrhea virus monoclonal antibody on the surface of Prussian blue/graphene modified electrode, drying, immersing in bovine serum albumin solution and reacting in a water bath to obtain the product. USE - The method is useful for preparing immunosensor for qualitatively and quantitatively detecting Porcine epidemic diarrhea virus (claimed). ADVANTAGE - The method enables preparation of immunosensor which has excellent sensitivity, accuracy and stability. DETAILED DESCRIPTION - Method for preparing an immunosensor involves (a) dropping 1 mg/ml graphene oxide dispersion to the surface of the electrode substrate, drying at room temperature, immersing the electrode substrate in 0.1 mol/l potassium chloride solution and scanning the electrode substrate using cyclic voltammetry at a scan rate of 100 mV/second for 20 turns in the potential range of -1.5 V to 0.5 V for degrading graphene modified electrode, (b) immersing the degraded graphene modified electrode obtained in step (a) in 0.1 mol/l Prussian blue solution, scanning the electrode using cyclic voltammetry at a scan rate of 50 mV/second for 60 turns in the potential range of 0-1 V for depositing Prussian blue layer to obtain the graphene/Prussian blue modified electrode and (c) dropping the Porcine epidemic diarrhea virus monoclonal antibody on the surface of Prussian blue/graphene modified electrode obtained in step (b), drying at room temperature, immersing in 1-2% bovine serum albumin solution and reacting in a water bath for 0.5-1 hour to obtain the product. INDEPENDENT CLAIMS are included for the following: (1) immunosensor prepared by performing the above-mentioned method; and (2) use of the immunobiosensor in detecting Porcine epidemic diarrhea virus.