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  • 专利标题:   Lotus protocorm-like bodies effect vitrification cryopreservation by placing lotus protocorm-like bodies on pre-culture medium, soaking in vitrification solution including graphene quantum dot, and storing in liquid nitrogen.
  • 专利号:   CN104255710-A, CN104255710-B
  • 发明人:   CHEN G, REN L, ZHANG D, ZHANG Y, WANG L
  • 专利权人:   UNIV SHANGHAI JIAOTONG
  • 国际专利分类:   A01H004/00, A01N003/00
  • 专利详细信息:   CN104255710-A 07 Jan 2015 A01N-003/00 201521 Pages: 8 Chinese
  • 申请详细信息:   CN104255710-A CN10468248 15 Sep 2014
  • 优先权号:   CN10468248

▎ 摘　　要

NOVELTY - Lotus protocorm-like bodies (PLB) effect vitrification cryopreservation comprises placing lotus PLB in a pre-culture medium for 1-5 days; soaking lotus PLB in loading solution for 40-60 minutes, and removing the loading solution; soaking the lotus PLB in a vitrification solution at 0-25 degrees C for 40-60 minutes; and storing lotus PLB in liquid nitrogen. The vitrification solution includes 0.1-0.5 g/L graphene quantum dot. USE - Method for lotus protocorm-like bodies (PLB) effect vitrification cryopreservation (claimed).