• 专利标题:   Amplification primer used in kit for rapid detection of Salmonella, is designed based on specific conserved region of Salmonella bcfC gene including forward primer bcf-F and reverse primer bcf-R, where forward primer bcf-F and reverse primer bcf-R has seventeen nucleotide sequence.
  • 专利号:   CN115851990-A
  • 发明人:   REN X, ZHAO B, XIE H, SUN L, SONG C, WU J, SHI Y, SHEN Q, ZHANG Y, GONG J, ZHU M, YANG J, ZHUANG L
  • 专利权人:   JIANGSU AGRIC FORESTRY VOCATIONAL COLL
  • 国际专利分类:   C12N015/11, C12Q001/6844, C12Q001/689, C12R001/42
  • 专利详细信息:   CN115851990-A 28 Mar 2023 C12Q-001/689 202334 Chinese
  • 申请详细信息:   CN115851990-A CN11004723 22 Aug 2022
  • 优先权号:   CN11004723

▎ 摘  要

NOVELTY - Amplification primer is designed based on specific conserved region of SalmonellabcfC gene including forward primer bcf-F and reverse primer bcf-R, where forward primer bcf-F and reverse primer bcf-R has 17 nucleotide sequence (SEQ ID NOS: 1 and 2), given in the specification. USE - Amplification primer used in kit for rapid detection of Salmonella (claimed). ADVANTAGE - The amplification primer completes whole process in 30 minutes, has simple, fast and sensitive operation and reliable results. DETAILED DESCRIPTION - Amplification primer is designed based on specific conserved region of Salmonella bcfC gene including forward primer bcf-F and reverse primer bcf-R, where forward primer bcf-F and reverse primer bcf-R has 17 nucleotide sequence (SEQ ID NOS: 1 and 2), which are ccgccgtgccgattcta and tcagccgcccttctcgt. INDEPENDENT CLAIMS are included for: a kit comprises amplification primers for rapid detection of Salmonella; and a method for rapidly detecting Salmonella by using amplification primer for rapid detection of Salmonella or kit to detect Salmonella by using a fluorescence detection method.