▎ 摘　　要

NOVELTY - A method for preparing carbon nanocomposite surface-enhanced Raman spectroscopy (SERS) substrate film involves adding trace graphene oxide (GO) to cellulose nanocrystal (CNC) solution to synthesize CNC-GO-silver nanoparticle nanocomposite by in-situ reduction method, and combining the synthesized nanocomposite with bacterial cellulose membrane (BCM) by small-dose multiple spin coating method to form the carbon nanocomposite SERS substrate film. USE - The carbon nanocomposite SERS substrate film is useful in detection of serum samples of cancer group and non-cancer group (claimed), and in blood analysis and detection. ADVANTAGE - The prepared film exhibits good SERS uniformity and sensitivity to Nile Blue A (NBA), and effectively improves disadvantages that silver-loaded cellulose nanocrystal substrate are easy to agglomerate and not easy to carry. The signal fluctuation of blood analysis is reduced, and the fluorescence interference in the Raman detection process is effectively weakened. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are included for: a carbon nanocomposite SERS substrate film prepared by the above method; and an application of the carbon nanocomposite SERS substrate film in detection of serum samples of cancer group and non-cancer group.