• 专利标题:   Deoxyribonucleic acid fluorescence detection involves adding carboxy-fluorescein-labeled single-stranded deoxyribonucleic acid and target strand deoxyribonucleic acid to buffer, adding reduced graphene oxide, reacting and detecting.
  • 专利号:   CN105300950-A
  • 发明人:   CHEN W, LIU Y, WANG Y, DENG H, HONG G
  • 专利权人:   UNIV FUJIAN MEDICAL
  • 国际专利分类:   C01B031/04, G01N021/64
  • 专利详细信息:   CN105300950-A 03 Feb 2016 G01N-021/64 201647 Pages: 10 English
  • 申请详细信息:   CN105300950-A CN10852411 29 Nov 2015
  • 优先权号:   CN10852411

▎ 摘  要

NOVELTY - Deoxyribonucleic acid fluorescence detection involves adding 6-carboxy-fluorescein-labeled single-stranded deoxyribonucleic acid and target strand deoxyribonucleic acid to tris-hydrochloric acid buffer, adding partially reduced graphene oxide, reacting at room temperature and detecting at fluorescence intensity of 520 nm and excitation wavelength of 494 nm. The partially reduced graphene oxide is obtained by mixing 4 mg/ml aqueous graphene oxide and 10 mmol/L sodium hydroxide, stirring, centrifuging, separating, washing using purified water and drying. USE - Deoxyribonucleic acid fluorescence detection (claimed). ADVANTAGE - The method enables simple and rapid deoxyribonucleic acid fluorescence detection with high sensitivity, at reduced dosage of partially reduced graphene oxide. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is included for kit for deoxyribonucleic acid fluorescence detection.