▎ 摘　　要

NOVELTY - Preparing multi-recognition site print sensor based on step-based self-assembly comprises pre-treating glass-carbon electrode, reducing graphene oxide modified electrode, nano- gold modified electrode and self-assembling. (a) pre-processing the glassy carbon electrode polishing and washing, placing the electrode in the H2SO4 solution containing chloric acid hydrate, depositing for 500-700s at -0.3-0.1V constant potential, taking out the electrode, sequentially ultra-pure water and absolute ethyl alcohol to wash, and naturally drying at room temperature to obtain AuNPs @rG@GCE to obtain C, (b) self-assembling composite functional monomers and template molecules separately to mix a functional monomer solution containing sulfhydryl and template molecule solution uniformly, (c) placing the E in the ethanol-NaOH water solution to induce and elute the template molecule for 10-20 minutes, and repeatedly washing by water to obtain corn erythritol molecular imprinting electrochemical sensor. USE - Preparing multi-recognition site print sensor based on step-based self-assembly for fast analyzing and measuring residual of corn zearalenol. ADVANTAGE - The method is simple, easy to control, and provides molecular imprinting electrochemical sensor with good stability, high sensitivity, low cost and fast response. The sensor prepared by the method is suitable for fast analyzing and measuring the residual of corn zearalenol in the sample. The method avoids the association effect between the composite functional monomer, reduces the yield of the by-product, and increases the effective identification site. The specificity of the target substance recognition is further increased. The self-assembly preparation of the sulphur gold system is simple and easy to form film, with better stability and order. The nano-gold particle has good conductivity and stable biological compatibility. The active molecule is spontaneously adsorbed on the heterophase interface by chemical bond to form ordered molecule assembly system, which is mainly based on the strong chemical