• 专利标题:   Electrodes based on reduced graphene oxides for viral detection applications, has film created from dip coating is activated with complementary deoxyribonucleic acid biomimetic structures, and microfluidic with ribonucleic acid (RNA) covers electrodes and allows viral electrochemical detection.
  • 专利号:   BR102021013981-A2
  • 发明人:   MELVA CHAMPI FARFAN A, FONTELLES OBELENIS F, PRADO J M Z, CHALLHUA REYNOSO R V, AKASHI L
  • 专利权人:   UNIV FUNDACAO FEDERAL DO ABC, VRS PESQUISA DESENVOLVIMENTO EIRELI
  • 国际专利分类:   C12Q001/68, G01N033/487, G01N033/569
  • 专利详细信息:   BR102021013981-A2 24 Jan 2023 G01N-033/569 202317 Pages: 28
  • 申请详细信息:   BR102021013981-A2 BR10013981 15 Jul 2021
  • 优先权号:   BR10013981

▎ 摘  要

NOVELTY - The electrode has a working electrode (ET) that is coated by layer of reduced graphene oxide (rGO) for detection of DNA particles and viral RNA. The working electrode is prepared with rGO solution. The deposition of a gold film is provided on copper electrodes by sputtering technique and formation of plate with electrodes contains overlapping films. The assembly of an amperometric cell with ET coated is provided with a layer of graphene oxides and activated with complementary DNA biomimetic structures (cDNA) and RNA. The film created from dip coating is activated with cDNA. The microfluidic with RNA covers the electrodes, and allows the viral electrochemical detection. The calibration of the system with reference electrode (ER) (20), control electrode (EC) (30) and working electrode (ET) (10) of rGO sensitized with cDNA is performed in a circuit that has a potentiostat (800) in which the measurements of cyclic voltammetry and chronoamperometry are collected to obtain the viral signal. USE - Electrodes based on reduced graphene oxides for viral detection applications. ADVANTAGE - The use of material allows to work with the detection of DNA particles and viral RNA in very small concentrations, and allows the detection of several viruses and other viruses. DESCRIPTION OF DRAWING(S) - The drawing shows a schematic view of the electrodes based on reduced graphene oxides for viral detection applications. 10Working electrode 20Reference electrode 30Control electrode