▎ 摘　　要

NOVELTY - Detection of phosphatidylinositol proteoglycan-3 (GPC-3) involves immersing gold nanoparticles-SPCE electrode with glutaraldehyde, washing with phosphate buffer solution (PBS), blow drying, adding reduced graphene oxide (RGO)-Hemin suspension to obtain RGO-Hemin/gold nanoparticles/SPCE electrode, adding aminated GPC-3 aptamer, incubating, adding bovine serum albumin solution to obtain GPC-3-Apt/RGO-hemin/gold nanoparticles/SPCE sensing interface, drawing the working curve of GPC-3, adding standard GPC-3 solution to the obtained GPC-3-Apt/RGO-hemin/gold nanoparticles/SPCE sensing interface, incubating to obtain working electrode, and drying, placing the working electrode in the PBS solution, testing different concentrations of GPC-3, drawing a standard curve, detecting GPC-3 in actual serum samples, and calculating the product content in the sample. USE - Detection of phosphatidylinositol proteoglycan-3 based on reduced graphene oxide-hemin-gold nano-composite material. ADVANTAGE - The method enables simple and economical detection of phosphatidylinositol proteoglycan-3 based on reduced graphene oxide-hemin-gold nano-composite material, with low detection limit in short period of time. DETAILED DESCRIPTION - Detection of GPC-3 based on reduced graphene oxide (RGO)-hemin-gold nano composite material involves preparing (i) reduced graphene oxide-hemin material by adding graphene oxide into distilled water and ultrasonically-dissolving to obtain aqueous solution of graphene oxide, adding heme to ammonia to dissolve it and adding a small amount of distilled water to make hemin solution, mixing hemin with RGO, adding hydrazine hydrate to the mixture of RGO and hemin for reduction after the water bath reaction, centrifuge to obtain RGO-hemin material, modifying (ii) electrode and constructing biosensing interface by placing screen-printed electrode in sulfuric acid solution and perform cyclic voltammetry to obtain the activated screen-printed electrode, washing with water to obtain the activated screen-printed electrode, placing the activated screen-printed electrode (SPCE) into the chloroauric acid solution for constant potential deposition, washing the electrode with water to obtain gold nanoparticles-SPCE electrode, immersing the gold nanoparticles-SPCE electrode with glutaraldehyde, washing with phosphate buffer solution (PBS), and blow drying, then adding RGO-Hemin suspension in drops to incubate for a period of time, washing with PBS, and drying to obtain RGO-Hemin/gold nanoparticles/SPCE electrode and adding aminated GPC-3 aptamer and dripping it on the sensor interface, incubating for a period of time, washing the GPC-3 aptamer not fixed to the interface with PBS solution, and adding bovine serum albumin solution in drop to block to obtain GPC-3-Apt/RGO-hemin/gold nanoparticles/SPCE sensing interface, drying, drawing (iii) the working curve of GPC-3 by adding the standard GPC-3 solution in drops to the obtained GPC-3-Apt/RGO-hemin/gold nanoparticles/SPCE sensing interface, incubating for a period of time, washing with PBS solution to obtain the working electrode, and drying, placing the working electrode in the PBS solution, and using the differential pulse voltammetry (DPV) of the electrochemical workstation to record the peak current, testing different concentrations of GPC-3, drawing a standard curve, and calculating the minimum detection limit of the method, and detecting (iv) of GPC-3 in actual serum samples from the obtain GPC-3-Apt/RGO-hemin/gold nanoparticles/SPCE sensing interface, adding the actual serum sample to be tested, incubating for a period of time, and washing with PBS solution to obtain working electrode and drying, placing working electrode in the PBS solution, using the DPV scan of the electrochemical workstation to record its peak current, and calculating the GPC-3 in the actual serum sample.