▎ 摘　　要

NOVELTY - Detection of a target RNA virus in a sample involves (a) contacting the sample with a non-RNA oligonucleotide probe comprising a luminescent or fluorescent label, where the probe comprises a nucleotide sequence that is complementary to a target RNA sequence in the viral RNA, (b) contacting the sample with graphene oxide to adsorb non-hybridized probe onto the graphene oxide, and optionally a ribonuclease that specifically cleaves single-stranded RNA, and (c) detecting the presence or absence of the viral RNA by measuring the luminescence or fluorescence. USE - The method and kit are useful for detection of a target positive-sense or negative sense single-stranded RNA virus chosen from severe acute respiratory syndrome coronavirus (SARS-CoV), hepatitis C virus (HCV), hepatitis A virus (HAV), hepatitis E virus, dengue virus (DENV), West Nile virus, yellow fever virus, rubella virus, Ross River virus, Sindbis virus, Chikungunya virus, poliovirus, rotavirus, Borna disease virus, Lassa virus, Hanta virus, Ebola virus, Marburg virus, measles virus, mumps virus, rabies virus, influenza virus, and enterovirus 71 (EV71) in a sample (all claimed). ADVANTAGE - The turn-on fluorescence detection method avoids false negative results (as the ribonuclease cleaves non-hybridized target RNA stretches and non-target RNA) and enables simple, reliable, low-cost, rapid, highly sensitive and robust detection of RNA virus in a sample. The method satisfies the market needs, as analysis time is shortened from 30 minutes to 1 hour (or more) to not greater than 5 minutes and also serves as a YES/NO point-of-care diagnostic. DETAILED DESCRIPTION - Method for detection of a target RNA virus in a sample involves (a) contacting the sample suspected to contain the RNA virus or viral RNA with a non-RNA oligonucleotide probe comprising a luminescent or fluorescent label, where the probe comprises a nucleotide sequence that is complementary to a target RNA sequence in the viral RNA, under conditions that allow hybridization of the probe to the target sequence to form a probe:target sequence duplex hybrid when the target RNA sequence is present in the sample, (b) contacting the sample with graphene oxide to adsorb non-hybridized probe onto the graphene oxide, where the luminescence or fluorescence of the label is quenched and optionally a ribonuclease that specifically cleaves single-stranded RNA, and (c) detecting the presence or absence of the viral RNA in the sample by measuring the luminescence or fluorescence of the sample. An INDEPENDENT CLAIM is included for a kit which comprises ribonuclease that specifically cleaves single-stranded RNA, an oligonucleotide probe comprising luminescent or fluorescent label, where the probe comprises a nucleotide sequence that is complementary to a target RNA sequence in the viral RNA under hybridization conditions, and graphene oxide.