▎ 摘　　要

NOVELTY - Biomimetic immunosensor and method for detecting estrogen are claimed. The biomimetic immunosensor comprises substrate electrode, graphene which is modified, nanogold which is precipitated on the surface of the substrate electrode, and polypyrrole which is served as a skeleton, where template molecules and the estradiol molecules are embedded into a polypyrrole membrane, the estradiol is removed from the polypyrrole membrane in the process of eluting the template molecules, and cavities matched with the estradiol are obtained on the surface of the membrane. USE - The biomimetic immunosensor is useful for detecting estrogen (claimed). ADVANTAGE - The biomimetic immunosensor has high flexibility and recognition identity. The detection method is simple, and suitable for the enrichment analysis and detection of the estradiol in the severe environments, preferably high temperature and high pressure. Several cavities matched with the estradiol molecules are formed on the surface of the biomimetic immunosensor, and hence specific identification on the estradiol molecules can be performed in the complicate sample environment. Estradiol molecules are enriched on the surface of the sensor, and hence the estradiol can be detected through electrochemical analytic method.