▎ 摘　　要

NOVELTY - A nafion/hemoglobin/nitrogen doped graphene quantum dot modified electrode preparing method involves preparing ionic liquid carbon paste electrode by weighing graphite powder and N-hexylpyridinium hexafluorophosphate and adding liquid paraffin as a binder. Nafion/hemoglobin/nitrogen doped graphene quantum dot composite film modified electrode is prepared by preparing hemoglobin,N-GQDs solution, dropping N-GQDs solution onto CILE surface and allowing to air dry, taking Hb solution onto CILE and allow to air dry naturally, applying Nafion to the surface of electrode to obtain product. USE - Method for preparing nafion/hemoglobin/nitrogen doped graphene quantum dot modified electrode. DETAILED DESCRIPTION - A nafion/hemoglobin/nitrogen doped graphene quantum dot modified electrode preparing method involves preparing ionic liquid carbon paste electrode by weighing graphite powder and N-hexylpyridinium hexafluorophosphate and adding liquid paraffin as a binder, into the mortar, fully grinding, filling ground mixture into glass electrode tube with inner diameter of 4 mm, inserting copper wire as a guide wire, solid mixture in the glass electrode pipe and compacting to obtain CILE, using electrode surface before polished to a mirror surface. Nafion/hemoglobin/nitrogen doped graphene quantum dot composite film modified electrode is prepared by preparing 15 mg mL-1 hemoglobin, 0.6 mg mL-1 N-GQDs solution, using layered coating method, dropping 8 mu l 0.6 mg mL-1 N-GQDs solution onto the CILE surface and allowing to air dry naturally, taking 8 mu l Hb solution onto CILE and allow to air dry naturally, applying 6 mu l of 0.5% Nafion to the surface of the electrode to obtain product. INDEPENDENT CLAIMS are also included for the following: (1) a nafion/hemoglobin/nitrogen doped graphene quantum dot modified electrode obtained by the method (2) an electrocatalytic performance study of an electrochemical enzyme sensor with a nitrogen doped-graphene quantum dot modified electrode, in which electrocatalytic activity of trichloroacetic acid is studied using the constructed enzyme sensor.