▎ 摘　　要

NOVELTY - Preparing pancreatic cancer immunosensor based on gold electrodeposition and gold-doped silver/copper oxide-graphene nanosheets (CuO-GS) as marker comprises e.g. polishing glass carbon electrode, and cleaning, taking chloroauric acid as base solution, scanning, carrying out electrodeposition on the gold electrode surface, washing, and drying, continuously dropping pancreatic cancer markers capture antibody Ab1 solution on the electrode surface, washing, placing in refrigerator, and drying, continuously dropping bovine serum albumin solution on the electrode surface, and washing. USE - The immunosensor is useful for detecting pancreatic cancer tumor marker (claimed). ADVANTAGE - The immunosensor has excellent biocompatibility, high catalytic performance, high sensitivity and relatively wide detection range; and achieves detection limit of 1.5 fg/ml. DETAILED DESCRIPTION - Preparing pancreatic cancer immunosensor based on gold electrodeposition and gold-doped silver/copper oxide-graphene nanosheets (CuO-GS) as marker comprises (i) polishing glass carbon electrode having diameter of 3-5 mm with aluminum oxide polishing powder, and using ultra-pure water for cleaning; (ii) taking 0.8-1.5% chloroauric acid as base solution, scanning for 30 seconds under -0.2V voltage, carrying out electrodeposition on the gold electrode surface, washing with ultra-pure water, and drying; (iii) continuously dropping 4-6 mu l, 8-12 mu g/ml pancreatic cancer markers capture antibody Ab1 solution on the electrode surface, washing with ultra-pure water, placing in 4 degrees C refrigerator, and then drying; (iv) continuously dropping 3 mu l 0.5-2% bovine serum albumin solution on the electrode surface, sealing the non-specific active site of the electrode surface, using ultra-pure water to wash, placing in 4 degrees C refrigerator, and then drying; (v) dropping 4-6 mu l, 0.01-100 ng/ml tumor marker antigen standard solution with different concentrations on the electrode surface, using ultra-pure water to wash, placing in 4 degrees C refrigerator, and drying; and (vi) dropping 4-6 mu l, 1-3 mg/ml detection antibody hatching material gold doped silver/CuO-GS/Ab2 solution and coating on the electrode surface, and placing in 4 degrees C of refrigerator to dry. An INDEPENDENT CLAIM is also included for detecting pancreatic cancer tumor marker using the immunosensor, comprising (a) using an electrochemical work station for three-electrode system testing process, saturated calomel electrode as a reference electrode, molybdenum electrode as an auxiliary electrode, preparing sensor as working electrode, and using 10 ml, 40-60 mmol/l pH 5.6-8 phosphate buffer solution for testing; (b) using chronoamperometry method for detecting pancreatic cancer tumor marker, where input voltage is -0.4 V, sampling interval is 0.1 seconds, and running time is 400 seconds; and (c) after stabilizing background electric current, adding 10 ml, 50 mmol/l of pH 7.4 phosphate buffer solution for every 50 seconds, then injecting 10 mu l hydrogen peroxide solution (5-10 mol/l), and recording current change.