• 专利标题:   Electrochemical electrode for use in biosensor that is utilized in detecting kanamycin in food, comprises glassy carbon electrode modified with graphene polyaniline compound, and kanamycin-resistant antibody.
  • 专利号:   CN104237344-A
  • 发明人:   GUO Y, WANG Y, XU Y, WANG H, CUI J, CUI M, HUANG J, LI J, LIU S, QIU T, XU W
  • 专利权人:   UNIV JINAN
  • 国际专利分类:   G01N027/30
  • 专利详细信息:   CN104237344-A 24 Dec 2014 G01N-027/30 201521 Pages: 9 Chinese
  • 申请详细信息:   CN104237344-A CN10442491 02 Sep 2014
  • 优先权号:   CN10442491

▎ 摘  要

NOVELTY - An electrochemical electrode comprises glassy carbon electrode modified with graphene polyaniline compound from bottom to top, polyamide-amine dendrimer-macromolecular gold nanocompound complexes, and kanamycin-resistant antibody. The surface of the glassy carbon electrode is sealed with bovine serum albumin (BSA) and 10 adenine bases of single chain-DNA. USE - Electrochemical electrode for use in biosensor that is utilized in detecting kanamycin in food. ADVANTAGE - The electrochemical electrode is suitable for industrial production, can be prepared simply and cost-effectively, has stable property, good repeatability, and detects kanamycin in food rapidly with detection limits of 4.6x 10-6 mu g/l. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is also included for a method for preparing the electrochemical electrode, which involves washing a glassy carbon electrode, dropping graphene-polyaniline nanocompound onto surface of glassy carbon electrode, drying the glassy carbon electrode at room temperature, washing the glassy carbon electrode with secondary water, immersing glassy carbon electrode into polyamide-amine dendrimer-macromolecular gold nanocompound complexes for 3 hours, washing the glassy carbon electrode with secondary water, drying the carbon electrode, dropping kanamycin antibody in phosphate-buffered saline (PBS) buffer solution on the surface of the electrode under room temperature for overnight, and sealing surface of the glassy carbon electrode with 0.1% BSA and 10 adenine bases of single chain-DNA for 2 hours to obtain the finished product.