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  • 专利标题:   Nucleic acid detection method based on clustered regularly interspaced short palindromic repeat-Cas trans-cleavage and graphene field-effect transistor involves using graphene field effect transistor to amplify Cas12b trans-cleavage detection signal in solution to detect monkeypox virus nucleic acid.
  • 专利号:   CN115820932-A
  • 发明人:   PAN Y, ZHANG J, WEN X, QIU A, LIAO K, CHEN S, XU C, ZHANG S, WANG L, ZHANG H, LUO D, LIU Q
  • 专利权人:   SHENZHEN WANWU SENSING TECHNOLOGY CO LTD, UNIV HUAZHONG SCI TECHNOLOGY UNION SHE
  • 国际专利分类:   C12Q001/6844, C12Q001/70
  • 专利详细信息:   CN115820932-A 21 Mar 2023 C12Q-001/70 202334 Chinese
  • 申请详细信息:   CN115820932-A CN11275598 18 Oct 2022
  • 优先权号:   CN11275598

▎ 摘　　要

NOVELTY - Nucleic acid detection method based on CRISPR-Cas trans-cleavage and graphene field-effect transistor comprises using a graphene field effect transistor to amplify the Cas12b trans-cleavage detection signal in solution to detect monkeypox virus nucleic acid. USE - The method is useful for nucleic acid detection based on CRISPR-Cas trans-cleavage and graphene field-effect transistor. ADVANTAGE - The method realizes ultra-sensitive detection of target DNA, has short detection time and high sensitivity.