▎ 摘　　要

NOVELTY - Preparing a chemiluminescence sensor based on reduced graphene oxide quenching luminol-gold nanoparticles, involves reducing graphene oxide is obtained by reducing graphene oxide with dopamine, and weighing 0.05-0.1 g of graphene oxide into a 250 mL beaker, adding 50-100 mL ultrapure water to it, and ultrasound for 2-4 hours. The 0.2-0.4 g of dopamine is added into the beaker and continue to sonicated for 2-4 hours, and transferred the beaker to 80-90 degrees C oil bath for 2-4 hours. The obtained product is washed three times with ultrapure water, and finally centrifuged at 8000 revolutions/minute, and the separated precipitate is dried in a vacuum drying oven at 50-60 degrees C. The 0.01-0.05 g of reduced graphene oxide is weighed, and dispersed uniformly in 30-50 mL ultrapure water. USE - Method for preparing a chemiluminescence sensor based on reduced graphene oxide quenching luminol-gold nanoparticles used in the chemiluminescence detection of adenosine to realize the detection of adenosine. ADVANTAGE - The method enables to prepared a chemiluminescence sensor based on reduced graphene oxide quenching luminol-gold nanoparticles, that provides theoretical support for the further application of this method to the detection of clinical adenosine and other biomarkers. DETAILED DESCRIPTION - Preparing a chemiluminescence sensor based on reduced graphene oxide quenching luminol-gold nanoparticles, involves reducing graphene oxide is obtained by reducing graphene oxide with dopamine, and weighing 0.05-0.1 g of graphene oxide into a 250 mL beaker, adding 50-100 mL ultrapure water to it, and ultrasound for 2-4 hours. The 0.2-0.4 g of dopamine is added into the beaker and continue to sonicated for 2-4 hours, and transferred the beaker to 80-90 degrees C oil bath for 2-4 hours. The obtained product is washed three times with ultrapure water, and finally centrifuged at 8000 revolutions/minute, and the separated precipitate is dried in a vacuum drying oven at 50-60 degrees C. The 0.01-0.05 g of reduced graphene oxide is weighed, and dispersed uniformly in 30-50 mL ultrapure water. The 0.01-0.03 g of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide and 0.01-0.03 g of N-hydroxysuccinimide are added to the dispersion. The 0.01-0.02 millimol adenosine aptamer is added to the above dispersion and incubated for 2-4 hours at room temperature. The 2-4 mL of 0.05mol/L chloroauric acid solution is pipetted into a 250 mL conical flask, add ultrapure water to dilute to 100 mL, and heat to boiling, and under vigorous stirring, quickly added 1-3 mL of 0.05 mol/L luminol standard stock solution, continue heating until the solution turns to wine red, and after stopping heating, let the solution cool to room temperature to obtain luminol-gold nano solution, and putit in a refrigerator at 4 degrees C for later use. The 2-5 mL of the luminol-gold nanoparticle solution is weighed into a 10 mL centrifuge tube, added 0.02-0.05 millimol of complementary strand DNA to the test tube, and incubate for 2-4 h ours at room temperature. The 2-5 mL 0.01 mol/L of the reduced graphene oxide is pipetted, and adenosine aptamer dispersion is placed in a 15 mL centrifuge tube, and added 2-5 mL luminol-gold nanoparticles/complementary strand DNA solution to the test tube, and incubate for 2-4 hours at room temperature. The 2-5 mL of luminol- gold nanoparticles/complementary strand DNA-adenosine aptamer/reduced graphene oxide dispersion prepared added into a 25mL colorimetric tube, and pipetted 1~2mL adenosine test solution and added to the colorimetric tube. The adenosine molecule and adenosine aptamer bind together because of specific recognition. The luminol- gold nanoparticles are separated from the reduced graphene oxide, so the quenching effect of the reduced graphene oxide on the luminol-gold nanoparticles disappears, the chemiluminescence is restored, and the quantitative detection of adenosine molecules is realized.