▎ 摘　　要

NOVELTY - Nanoprobe comprising light-absorbing nanomaterials, polyethylene glycol, circulating tumor cell targets and fluorescent group-modified miRNAs single-stranded probes. The circulating tumor cell target is connected to the light-absorbing nanomaterial through the polyethylene glycol. The fluorescent group-modified miRNAs single-stranded probe is adsorbed on the surface of the light-absorbing nanomaterial. The binding force of the fluorescent group-modified miRNAs single-stranded probe with the target miRNAs is greater than the binding force of the fluorescent group-modified miRNAs single-stranded probe with the light-absorbing nanomaterial. USE - The nanoprobe is useful for preparing miRNAs products for detecting circulating tumor cells (claimed). ADVANTAGE - This nanoprobe: sensitively detects its miRNAs in single live circulating tumor cells (CTC) without enriching CTCs; and solves the technical defect that the existing miRNA methods for detecting CTCs are difficult to be enriched and easily cause invasive damage to CTCs. DETAILED DESCRIPTION - INDEPENDENT CLAIMS are also included for: (1) preparing nano probes, comprising (1) mixing and reacting polyethylene glycol and maleimide to obtain maleimidized polyethylene glycol, mixing and reacting the maleimidized polyethylene glycol with polypeptide capable of raking, and recognizing circulating tumor cells to obtain a polyethylene glycol polypeptide composite chain, (2) mixing the MOF nanosheet dispersion with the polyethylene glycol polypeptide composite chain, and preparing the MOF nanosheet-polyethylene glycol polypeptide composite chain after removing the precipitate, and (3) mixing and incubating the fluorescent group-modified miRNAs single-stranded probe with the MOF nanosheet-polyethylene glycol polypeptide composite chain to prepare nanoprobe; and (2) detection system for miRNAs in circulating tumor cells.