▎ 摘　　要

NOVELTY - Preparing targeted drug carrier involves (a) adding e.g. flake graphite powder to cooled container, and ultrasonically processing, (b) adding with potassium permanganate, (c) reacting mixture, (d) adding with water, and stirring reaction, (e) adding hydrogen peroxide solution, and stirring reaction, (f) adding hydrochloric acid solution, (g) centrifuging, and freeze-drying liquid, (h) pulverizing graphene oxide sheet, (i) adding dichloromethane to another container, (j) adding to diethylamino sulfur trifluoride and ultrasonically processing, (k) adding methanol, filtering and drying mixture, (l) ultrasonically processing fluorinated graphene, (m) adding to e.g. sodium hydroxide, (n) centrifuging and freeze-drying mixture, (o) dispersing fluorinated graphene in dimethyl sulfoxide, (p) dissolving polyethyleneimine (PEI), adding to graphene solution and freeze-drying, (q) dissolving hyaluronic acid in water, (r) dispersing graphene-PEI, (s) dialyzing solution and (t) drying drug carrier. USE - The method is useful for preparing targeted drug carrier. ADVANTAGE - The method prepares targeted drug carrier with improved biocompatibility. DETAILED DESCRIPTION - Method for preparing a targeted drug carrier based on hyaluronic acid modified fluorinated graphene involves (a) cooling a container to 0-5 degrees C in an ice water bath, adding 20-25 ml concentrated sulfuric acid to the container, adding 0.5-2 g flake graphite powder and 0.2-1 g sodium nitrate to container, and ultrasonically processing for 20-30 minutes, (b) stirring the reaction, maintaining the reaction temperature at below 10 degrees C, weighing 2-4 g potassium permanganate, adding it in batches within 25-30 minutes, and reacting for 1.5-2.5 hours, (c) raising the temperature to 35-40 degrees C, and performing the reaction for 25-30 minutes, (d) measuring 70-120 ml water, adding it after cooling to 0-5 degrees C in an ice water bath, adjusting the temperature to 90-95 degrees C, and continuously stirring the reaction for 25-30 minutes, (e) adding 50-70 ml water to stop the reaction, adding 25-35 vol.% of 20-30 ml hydrogen peroxide (H2O2) solution, and stirring reaction for 15-20 minutes, (f) adding 30-50 ml of 8-12 vol.% hydrochloric acid (HCl) solution, and maintaining mixture for 4-5 days, (g) removing the supernatant of the solution, centrifuging the viscous liquid until the viscous liquid is neutral, and freeze-drying the viscous liquid for 24-48 hours to obtain graphene oxide sheet, (h) pulverizing the graphene oxide sheet into graphene oxide powder, (i) adding 0.5-1.5 g graphene oxide powder, and 20-40 ml dichloromethane or 1,2-dichloroethane to another container, and stirring for 10-14 hours to make it evenly dispersed, (j) adding dropwise 0.5-1 ml diethylamino sulfur trifluoride, ultrasonically processing at room temperature for 4-6 hours, and stirring the reaction for 24-72 hours, (k) adding 15-30 ml methanol to terminate the reaction, filtering to obtain a portion of the fluorinated graphene product, fully washing it with ethanol and deionized water, and drying at 50-70 degrees C, (l) weighing 25-30 mg partially fluorinated graphene in 45-60 ml water, and ultrasonically processing to obtain a partially fluorinated graphene solution, (m) taking 1-1.5 g sodium hydroxide and 1.2-1.5 g sodium hypochlorite, adding portion of the fluorinated graphene solution, and ultrasonically processing for 3-4 hours, (n) centrifuging to remove the supernatant, adding water to obtain a uniform dispersion, adding dilute hydrochloric acid until the pH is neutral, placing in water for 48-72 hours, and freeze-drying mixture, (o) weighing 20-30 mg fluorinated graphene, dispersing it in 5-10 ml dimethyl sulfoxide (DMSO), and adding 10-15 ml 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride to activate the stirring reaction for 3-5 hours to obtain an activated fluorinated graphene solution, (p) dissolving 35-40 mg polyethyleneimine (PEI) in 15-25 ml DMSO, dropwise adding obtained PEI solution to the activated fluorinated graphene solution, stirring reaction at room temperature under ultrasonic stirring for 24-36 hours, removing unreacted impurities by dialysis, and freeze-drying to obtain fluorinated graphene-PEI, (q) weighing 0.1-0.12 g hyaluronic acid, dissolving it in 5-10 ml water, adding 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide to activate the stirring reaction for 3-5 hours to obtain an activated hyaluronic acid solution, (r) weighing 20-30 mg fluorinated graphene-PEI, dispersing it in 5-15 ml water, adding resulting fluorinated graphene-PEI solution to the activated hyaluronic acid solution, stirring the reaction at room temperature for 24-36 hours, and removing unreacted impurities by dialysis, (s) dialyzing the solution using a sodium bicarbonate solution having a pH of 7.8-8.5, changing the water every 3-4 hours, transferring it to water after 48-60 hours, and dialyzing for 24-36 hours, and (t) removing water from the solution using rotary evaporation, and drying targeted drug carrier.