▎ 摘　　要

NOVELTY - The dual target aptamer sensor for simultaneous detection of aflatoxin B1 (AFB1) and ZEN, has: AFB1 aptamer DNA1, ZEN aptamer DNA2 and gold electrode. The DNA1 is thionine (Thi), and nano-gold is marked to form DNA1-AuNPs-Thi aptamer functional probe. The DNA2 is 6- (ferrocenyl) hexanethiol (FC6S), and nano-gold to marked to form DNA2-AuNPs-FC6S aptamer functional probe. Molybdenum disulfide-reducible graphene oxide is introduced on the surface of gold electrode, and the two target aptamer functional probe after the mark is fixed in the electrode surface through gold sulfur bond. USE - The double-target aptamer sensor is useful for simultaneously detecting AFB1 and ZEN in agricultural products, preferably grain and oil crops. ADVANTAGE - The aptamer sensors for simultaneously detecting AFB1 and ZEN are constructed by respectively labeling AFB1 aptamer (DNA1) and ZEN aptamer (DNA2) with Thi and FC6S, utilizing obvious DPV peaks generated by electroactive substances Thi and FC6S at different potentials, and introducing molybdenum disulfide-reduced graphene oxide on the surface of the gold electrode nanomaterial, which enhances the biocompatibility, specific surface area and conductivity of the working electrode, thus increasing the immobilization amount of the aptamer functionalized probe on the surface of the working electrode, and providing an interface with good biocompatibility for the combination of the aptamer and the target. Simultaneously the good conductivity further enhances the current change generated when the aptamer and the target are combined, so that the aptamer sensor provided by the application has good sensitivity, stability, reproducibility and interference resistance to AFB1 and ZEN, and the lowest detection limit of AFB1 and ZEN also reaches 0.0003 ng. The method can be applied to the simultaneous detection of AFB1 and ZEN in the actual edible oil sample, and the standard addition recovery rate is good. The sensor construction method can be used for detecting other different types of mycotoxins by replacing different identification elements (other types of aptamers), thus popularizing the detection technology and application in a wider range and providing technical support for quickly and sensitively detecting various mycotoxins. DETAILED DESCRIPTION - An INDEPENDENT CLAIM is included for a method of making an aptamer sensor for simultaneous detection of AFB1 and ZEN. DESCRIPTION OF DRAWING(S) - The drawing shows an aptamer sensor construction process;